To shed more light on whether OXPHOS defects can play a role in the development of heart failure, we analyse samples from patients undergoing heart transplants. We search for characteristic markers, which would be suitable for identification of new patients, as well as new potential targets for treatment.
Chronic heart failure is one of the civilization diseases that is spreading in the developed countries. There are two reasons – aging of the population and a higher incidence of metabolic risks (obesity and diabetes). Therefore, the topics current research focuses on include studying the developmental mechanisms of heart failure and searching for new treatment procedures. Defects of energy production in mitochondria represent a very important factor because the heart has extremely high demands for energy supply.
Our new project in collaboration with the Institute of Clinical and Experimental Medicine (IKEM, Prague) is aimed to clarify how often abnormalities of mitochondrial energy-producing apparatus are present in the myocardium of patients suffering from developed heart failure. We also want to study mechanisms that lead to its development. Specifically, we are going:
In the case of patients who lived with the ventricular assist device, we are going to study the effects of this treatment on mitochondrial bioenergetics.
The OROBOROS oxygraph for measurements of oxygen consumption by heart mitochondria is an important method for analysis of mitochondrial function in the heart.