Animal species: mouse, rat, dog, cat, horse, ferret
Measured parameters: WBC, RBC, HGB, HCT, MCV, MCH, MCHC, RDW, PLT, MPV, LYM, MON, GRA, EOS
Animal species: rabbit, pig, sheep, cattle
Measured parameters: WBC, RBC, HGB, HCT, MCV, MCH, MCHC, PLT
Minimum sample volume: 50 μl EDTA blood
Blood measurement should be done within 4 hours after blood collection
Technical specifications
Method: RBC, WBC and PLTS- electrical impedance, hemoglobin - spectral photometry
scil Vet abc Plus+: Analytical instrument to determine hematological parameters from whole blood
Technical details
Animal species: rat
Measured parameters: APTT, PT
Minimum sample volume: 60 μl blood citrate plasma
Rayto RT-2204 C Analytical instrument to determine coagulation parameters from blood plasma
Venous blood should be carefully collected into a tube treated by sodium citrate solution. Attention should be paid to maintain of ratio of 9 parts blood to 1 part citrate. Tubes with blood should be mixed well and centrifuged (15 minutes, 2500 g, 20 °C) as soon as possible after blood collection. Plasma samples should be maintained at room temperature and should be tested within 4 hours by PT test and within 8 hours by APTT test.
Untested residue of citrate plasma should be processed to platelet-free citrate plasma by second centrifugation (30 minutes, 3000 g, 20 °C). Separated platelet-free plasma should be immediately frozen at -80 °C for further possible testing.
Frozen platelet-free citrate plasma should be thawed at 37 °C for 15 minutes and then should be mixed well and centrifuged (5 minutes, 500 RPM, 20 °C). The plasma should be tested immediately after centrifugation.