{"id":22315,"date":"2017-11-14T10:19:01","date_gmt":"2017-11-14T09:19:01","guid":{"rendered":"https:\/\/fgu.antstudio.dev\/2017\/11\/objasneni-molekularni-podstaty-interakce-proteinu-14-3-3-aktivujiciho-trehalasu-nth1-v-bunkach-kvasinek\/"},"modified":"2017-11-14T10:19:01","modified_gmt":"2017-11-14T09:19:01","slug":"objasneni-molekularni-podstaty-interakce-proteinu-14-3-3-aktivujiciho-trehalasu-nth1-v-bunkach-kvasinek","status":"publish","type":"post","link":"https:\/\/fgu.cas.cz\/en\/2017\/11\/objasneni-molekularni-podstaty-interakce-proteinu-14-3-3-aktivujiciho-trehalasu-nth1-v-bunkach-kvasinek\/","title":{"rendered":"Objasn\u011bn\u00ed molekul\u00e1rn\u00ed podstaty interakce proteinu 14-3-3 aktivuj\u00edc\u00edho trehalasu Nth1 v bu\u0148k\u00e1ch kvasinek"},"content":{"rendered":"

V\u00fdzkumn\u00fd t\u00fdm Veroniky Ob\u0161ilov\u00e9 z Fyziologick\u00e9ho \u00fastavu AV \u010cR a Tom\u00e1\u0161e Ob\u0161ila z P\u0159\u00edrodov\u011bdeck\u00e9 fakulty UK objasnil mechanismus, kter\u00fdm b\u00edlkovina 14-3-3 reguluje funkci kvasni\u010dn\u00e9ho enzymu neutr\u00e1ln\u00ed trehalasy Nth1.<\/strong><\/p>\n

Neutr\u00e1ln\u00ed trehalasa Nth1 katalyzuje hydrol\u00fdzu disacharidu trehalosy a p\u0159edstavuje jeden z kl\u00ed\u010dov\u00fdch enzym\u016f energetick\u00e9ho metabolismu kvasinek. V\u00fdsledky jejich v\u00fdzkumu, kter\u00fd prob\u00edhal v\u00fdhradn\u011b v \u010cesk\u00e9 Republice, nyn\u00ed publikoval presti\u017en\u00ed americk\u00fd v\u011bdeck\u00fd \u010dasopis Proceedings of the National Academy of Sciences of the USA (PNAS). \u201eVy\u0159e\u0161en\u00e1 atom\u00e1rn\u00ed struktura komplexu proteinu 14-3-3 s neutr\u00e1ln\u00ed trehalasou Nth1 ukazuje schopnost proteinu 14-3-3 ovliv\u0148ovat konformaci multidom\u00e9nov\u00e9ho enzymu a fungovat jako tzv. alosterick\u00fd regul\u00e1tor. Jedn\u00e1 se o teprve druhou zn\u00e1mou strukturu komplexu proteinu 14-3-3 s nav\u00e1zan\u00fdm pln\u011b funk\u010dn\u00edm enzymem,\u201c \u0159\u00edk\u00e1 Veronika Ob\u0161ilov\u00e1.<\/p>\n

V\u011bdc\u016fm z Prahy se poda\u0159ilo objasnit krystalov\u00e9 struktury n\u011bkolika forem enzymu neutr\u00e1ln\u00ed trehalasy Nth1 a popsat zm\u011bny, ke kter\u00fdm doch\u00e1z\u00ed v d\u016fsledku vazby ligandu a regula\u010dn\u00edho proteinu 14-3-3. Uk\u00e1zali, \u017ee enzymov\u00e1 aktivita Nth1 je podm\u00edn\u011bna spr\u00e1vnou vz\u00e1jemnou prostorovou orientac\u00ed katalytick\u00e9 a v\u00e1pn\u00edk-v\u00e1zaj\u00edc\u00ed dom\u00e9ny Nth1, kter\u00e1 umo\u017e\u0148uje stabilizaci katalytick\u00e9ho centra enzymu. Z\u00faro\u010dili tak dlouhodob\u00fd v\u00fdzkum zam\u011b\u0159en\u00fd na pochopen\u00ed mechanismu regulace kvasni\u010dn\u00e9 trehalasy.<\/p>\n

\u201ePoda\u0159ilo se n\u00e1m rozkl\u00ed\u010dovat strukturn\u00ed podstatu aktivace enzymu Nth1 pomoc\u00ed proteinu 14-3-3. Porovn\u00e1n\u00ed struktury komplexu 14-3-3:Nth1 s ostatn\u00edmi 14-3-3 komplexy potvrzuje, \u017ee proteiny 14-3-3 ovliv\u0148uj\u00ed strukturu a t\u00edm i funkci \u0159ady jin\u00fdch protein\u016f d\u016fle\u017eit\u00fdch pro regulaci kl\u00ed\u010dov\u00fdch d\u011bj\u016f v bu\u0148ce,\u201c vysv\u011btluje Veronika Ob\u0161ilov\u00e1.<\/p>\n

\"-\"<\/p>\n

Detailn\u00ed pohled na strukturu komplexu protein\u016f 14-3-3 a Nth1 (PDB 5M4A).<\/p>\n

Alblova M, Smidova A, Docekal V, Vesely J, Herman P, Obsilova V, Obsil T. Molecular basis of the 14-3-3 protein-dependent activation of yeast neutral trehalase Nth1. Proc Natl Acad Sci U S A. 2017 Nov 14;114(46):E9811-E9820. IF = 9.66.<\/p>\n","protected":false},"excerpt":{"rendered":"

V\u00fdzkumn\u00fd t\u00fdm Veroniky Ob\u0161ilov\u00e9 z Fyziologick\u00e9ho \u00fastavu AV \u010cR a Tom\u00e1\u0161e Ob\u0161ila z P\u0159\u00edrodov\u011bdeck\u00e9 fakulty UK objasnil mechanismus, kter\u00fdm b\u00edlkovina 14-3-3 reguluje funkci kvasni\u010dn\u00e9ho enzymu neutr\u00e1ln\u00ed trehalasy Nth1. Neutr\u00e1ln\u00ed trehalasa Nth1 katalyzuje hydrol\u00fdzu disacharidu trehalosy a p\u0159edstavuje jeden z kl\u00ed\u010dov\u00fdch enzym\u016f energetick\u00e9ho metabolismu kvasinek. V\u00fdsledky jejich v\u00fdzkumu, kter\u00fd prob\u00edhal v\u00fdhradn\u011b v \u010cesk\u00e9 Republice, nyn\u00ed publikoval presti\u017en\u00ed americk\u00fd v\u011bdeck\u00fd \u010dasopis […]<\/p>\n","protected":false},"author":1,"featured_media":22311,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"inline_featured_image":false,"footnotes":""},"categories":[2],"tags":[],"class_list":["post-22315","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-other"],"acf":[],"_links":{"self":[{"href":"https:\/\/fgu.cas.cz\/en\/wp-json\/wp\/v2\/posts\/22315","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/fgu.cas.cz\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/fgu.cas.cz\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/fgu.cas.cz\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/fgu.cas.cz\/en\/wp-json\/wp\/v2\/comments?post=22315"}],"version-history":[{"count":0,"href":"https:\/\/fgu.cas.cz\/en\/wp-json\/wp\/v2\/posts\/22315\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/fgu.cas.cz\/en\/wp-json\/wp\/v2\/media\/22311"}],"wp:attachment":[{"href":"https:\/\/fgu.cas.cz\/en\/wp-json\/wp\/v2\/media?parent=22315"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/fgu.cas.cz\/en\/wp-json\/wp\/v2\/categories?post=22315"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/fgu.cas.cz\/en\/wp-json\/wp\/v2\/tags?post=22315"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}