Liquid chromatography-mass spectrometry (LC-MS) is preferred for analyzing small molecules such as polar metabolites, complex lipids, and drugs in biofluids [1]. However, capturing the full range of these compounds often requires multiple extraction methods or platforms [2]. We optimized an LC-MS workflow for extracting polar metabolites, complex lipids, and drugs from human plasma, followed by rapid LC-MS analysis. The extraction process utilized a bi-phase approach with a methanol\/methyl tert-butyl ether mixture and water, enabling separate analyses of the organic phase (for complex lipids) and aqueous phase (for polar metabolites) for untargeted metabolomics and lipidomics [3]. To address the wide range of drug polarities, an additional extraction step using a methanol\/ethanol mixture was performed. Using MS-DIAL software and combined MS\/MS spectral libraries, we annotated over 600 complex lipids and polar metabolites from plasma samples of type 2 diabetes (T2D) patients and non-T2D controls. Targeted drug analysis quantified more than 40 drugs and selected metabolites at concentrations as low as 1\u201310 ng\/mL, providing sufficient sensitivity for monitoring drug adherence. This study highlights metabolome and lipidome alterations in non-adherent T2D patients and discusses the tools available for data interpretation.<\/p>\n
[1] Rakusanova S, Cajka T. Trend Anal Chem (2024) 180:117940.
\n[2] Rakusanova S, Cajka T. Trend Anal Chem (2023) 158:116831.
\n[3] Cajka T, et al. Int J Mol Sci (2023) 24(3) 1987.<\/p>\n
IPHYS contact person: Tom\u00e1\u0161 \u010cajka, tomas.cajka@fgu.cas.cz<\/p>\n","protected":false},"excerpt":{"rendered":"
Liquid chromatography-mass spectrometry (LC-MS) is preferred for analyzing small molecules such as polar metabolites, complex lipids, and drugs in biofluids [1]. However, capturing the full range of these compounds often requires multiple extraction methods or platforms [2]. We optimized an LC-MS workflow for extracting polar metabolites, complex lipids, and drugs from human plasma, followed by […]<\/p>\n","protected":false},"author":5,"featured_media":55280,"template":"","meta":{"_acf_changed":false,"inline_featured_image":false,"footnotes":""},"kategorie-udalosti":[84],"class_list":["post-38986","udalost","type-udalost","status-publish","has-post-thumbnail","hentry","kategorie-udalosti-lectures"],"acf":[],"_links":{"self":[{"href":"https:\/\/fgu.cas.cz\/en\/wp-json\/wp\/v2\/udalost\/38986","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/fgu.cas.cz\/en\/wp-json\/wp\/v2\/udalost"}],"about":[{"href":"https:\/\/fgu.cas.cz\/en\/wp-json\/wp\/v2\/types\/udalost"}],"author":[{"embeddable":true,"href":"https:\/\/fgu.cas.cz\/en\/wp-json\/wp\/v2\/users\/5"}],"version-history":[{"count":0,"href":"https:\/\/fgu.cas.cz\/en\/wp-json\/wp\/v2\/udalost\/38986\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/fgu.cas.cz\/en\/wp-json\/wp\/v2\/media\/55280"}],"wp:attachment":[{"href":"https:\/\/fgu.cas.cz\/en\/wp-json\/wp\/v2\/media?parent=38986"}],"wp:term":[{"taxonomy":"kategorie-udalosti","embeddable":true,"href":"https:\/\/fgu.cas.cz\/en\/wp-json\/wp\/v2\/kategorie-udalosti?post=38986"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}