{"id":49021,"date":"2025-09-22T21:46:39","date_gmt":"2025-09-22T19:46:39","guid":{"rendered":"https:\/\/fgu.cas.cz\/?post_type=udalost&#038;p=49021"},"modified":"2025-11-18T11:03:29","modified_gmt":"2025-11-18T10:03:29","slug":"metabolomics-and-lipidomics-platforms-pitfalls-and-practical-tips","status":"publish","type":"udalost","link":"https:\/\/fgu.cas.cz\/en\/event\/metabolomics-and-lipidomics-platforms-pitfalls-and-practical-tips\/","title":{"rendered":"Metabolomics and lipidomics: platforms, pitfalls, and practical tips"},"content":{"rendered":"<p>Metabolomics and lipidomics are rapidly advancing fields that have transformed our understanding of biological processes at the molecular level. Yet, designing an appropriate workflow and selecting from the many available analytical options can be challenging [1]. Since 2018, the Metabolomics Core Facility at the Institute of Physiology CAS (<a href=\"https:\/\/metabolomics.fgu.cas.cz\">https:\/\/metabolomics.fgu.cas.cz<\/a>) has offered fee-based services for analyzing polar metabolites, complex lipids, and exposome compounds (including drugs) in biological materials [2]. The scope of these services has steadily expanded, with approximately 5,000 samples analyzed annually using a multiplatform liquid chromatography\u2013mass spectrometry (LC\u2013MS) approach. This lecture will present the current untargeted LC\u2013MS platforms (LIMeX workflow) and introduce upcoming platforms. We will also discuss common pitfalls related to study design, metabolome coverage, and statistical analysis.<\/p>\n<p><strong>References:<\/strong><\/p>\n<p>[1] Rakusanova &amp; Cajka, <em>Trends Anal Chem<\/em> 180 (2024) 117940 (doi: <a href=\"https:\/\/doi.org\/10.1016\/j.trac.2024.117940\" target=\"_blank\" rel=\"noopener\">10.1016\/j.trac.2024.117940<\/a>)<\/p>\n<p>[2] Cajka et al., <em>Int J Mol Sci<\/em> 324 (2023) 1987 (doi: <a href=\"https:\/\/doi.org\/10.3390\/ijms24031987\" target=\"_blank\" rel=\"noopener\">10.3390\/ijms24031987<\/a>)<\/p>\n<p><strong>IPHYS contact person: <\/strong><a href=\"https:\/\/fgu.cas.cz\/en\/profil\/doc-ing-tomas-cajka-ph-d\/\">doc. Ing. Tom\u00e1\u0161 \u010cajka, Ph.D.,<\/a> Laboratory of Translational Metabolism, Metabolomics department, tomas.cajka@fgu.cas.cz<\/p>\n<p><strong>Funding: <\/strong>Output of project no. LX22NPO5104 \u201eFunded by the European Union &#8211; Next Generation EU\u201c<\/p>\n<h1 class=\"elementor-heading-title elementor-size-default\"><\/h1>\n","protected":false},"excerpt":{"rendered":"<p>Metabolomics and lipidomics are rapidly advancing fields that have transformed our understanding of biological processes at the molecular level. Yet, designing an appropriate workflow and selecting from the many available analytical options can be challenging [1]. Since 2018, the Metabolomics Core Facility at the Institute of Physiology CAS (https:\/\/metabolomics.fgu.cas.cz) has offered fee-based services for analyzing [&hellip;]<\/p>\n","protected":false},"author":11,"featured_media":55280,"template":"","meta":{"_acf_changed":false,"inline_featured_image":false,"footnotes":""},"kategorie-udalosti":[139],"class_list":["post-49021","udalost","type-udalost","status-publish","has-post-thumbnail","hentry","kategorie-udalosti-biocev-krc-methodological-seminars-en"],"acf":[],"_links":{"self":[{"href":"https:\/\/fgu.cas.cz\/en\/wp-json\/wp\/v2\/udalost\/49021","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/fgu.cas.cz\/en\/wp-json\/wp\/v2\/udalost"}],"about":[{"href":"https:\/\/fgu.cas.cz\/en\/wp-json\/wp\/v2\/types\/udalost"}],"author":[{"embeddable":true,"href":"https:\/\/fgu.cas.cz\/en\/wp-json\/wp\/v2\/users\/11"}],"version-history":[{"count":0,"href":"https:\/\/fgu.cas.cz\/en\/wp-json\/wp\/v2\/udalost\/49021\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/fgu.cas.cz\/en\/wp-json\/wp\/v2\/media\/55280"}],"wp:attachment":[{"href":"https:\/\/fgu.cas.cz\/en\/wp-json\/wp\/v2\/media?parent=49021"}],"wp:term":[{"taxonomy":"kategorie-udalosti","embeddable":true,"href":"https:\/\/fgu.cas.cz\/en\/wp-json\/wp\/v2\/kategorie-udalosti?post=49021"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}