{"id":30122,"date":"2024-08-23T20:06:19","date_gmt":"2024-08-23T18:06:19","guid":{"rendered":"https:\/\/fgu.antstudio.dev\/?post_type=vyzkumny-projekt&#038;p=30122"},"modified":"2024-09-02T08:08:35","modified_gmt":"2024-09-02T06:08:35","slug":"involvement-of-the-adiponectin-ampk-axis-in-insulin-sensitizing-effects-of-thiazolidinediones-colncluded-project","status":"publish","type":"vyzkumny-projekt","link":"https:\/\/fgu.cas.cz\/en\/research-project\/involvement-of-the-adiponectin-ampk-axis-in-insulin-sensitizing-effects-of-thiazolidinediones-colncluded-project\/","title":{"rendered":"Involvement of the adiponectin-AMPK axis in insulin-sensitizing effects of thiazolidinediones (colncluded project)"},"content":{"rendered":"<div>\n<p><strong>Thiazolidinediony (TZD) jsou \u0161iroce pou\u017e\u00edv\u00e1ny p\u0159i l\u00e9\u010db\u011b inzulinov\u00e9 resistence u pacient\u016f s&nbsp;diabetes mellitus 2.&nbsp;typu (DM). Tato l\u00e9\u010dba je v\u0161ak spojena s&nbsp;n\u011bkter\u00fdmi ne\u017e\u00e1douc\u00edmi \u00fa\u010dinky. Je proto zapot\u0159eb\u00ed nov\u00fdch l\u00e9\u010debn\u00fdch postup\u016f, kter\u00e9 by umo\u017enily pou\u017e\u00edt ni\u017e\u0161\u00edch (suboptim\u00e1ln\u00edch) d\u00e1vek TZD v&nbsp;kombinaci s&nbsp;dal\u0161\u00edmi farmakologick\u00fdmi \u010di dietetick\u00fdmi intervencemi.<\/strong><\/p>\n<\/div>\n<p>In our previous studies\u00a0<a href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/19277604\" target=\"_blank\" rel=\"noopener\">(Kuda et al. 2009. Diabetologia<\/a>;\u00a0<a href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/22952760\" target=\"_blank\" rel=\"noopener\">Horakova et al. 2012. PLoS One<\/a>;\u00a0<a href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/22073272\" target=\"_blank\" rel=\"noopener\">Kus et al. 2011. PLoS One<\/a>), we showed a potential of omega-3 to enhance the effect of sub-optimal doses of TZDs.<\/p>\n<p align=\"left\">\n<p>The insulin-sensitizing effect of TZDs is thought to results from activation of PPAR\u03b3, associated with the induction of adiponectin and stimulation of AMP-activated protein kinase (<strong>AMPK<\/strong>). However, our data suggest that the mechanism underlying effects of sub-optimal doses of TZDs on insulin sensitivity may depend on a change of plasma lipid profile resulting from modulation of hepatic lipid metabolism by TZDs, independent of the adiponectin-AMPK axes. The goal of this project is to characterize the dose-dependent mechanism of TZDs action using mice with genetically disrupted AMPK. Characterization of the mechanisms behind the dose-dependent effects TZDs will allow for designing novel combination treatment strategies for DM patients, while reducing both the risks and the cost of the TZD therapy.<\/p>\n<p align=\"left\">\n<p><em>Supported by the\u00a0<\/em><em>Project CSF (no. P301\/11\/0226;\u00a0<\/em><em>2011-2015) PI:\u00a0 Pavel Flachs, PhD; collaborator: Eva Tvrzicka PhD; 1<sup>st<\/sup>\u00a0Medical Faculty, Charles University, Prague.<\/em><\/p>\n<p>&nbsp;<\/p>\n<p><img fetchpriority=\"high\" decoding=\"async\" class=\"size-full wp-image-27069 aligncenter\" src=\"https:\/\/fgu.cas.cz\/wp-content\/uploads\/2024\/08\/2014-03-01-13-35-381.png\" alt=\"-\" width=\"604\" height=\"452\" title=\"\" srcset=\"https:\/\/fgu.cas.cz\/wp-content\/uploads\/2024\/08\/2014-03-01-13-35-381.png 604w, https:\/\/fgu.cas.cz\/wp-content\/uploads\/2024\/08\/2014-03-01-13-35-381-300x225.png 300w\" sizes=\"(max-width: 604px) 100vw, 604px\" \/><\/p>\n<p><em>In vitro experiments are performed to complement animal studies. Preadiopocytes and differentiated adipocytes in cell culture with lipid droplets stained using Oil red are shown.<\/em><\/p>\n<p style=\"text-align: center;\"><em>\u00a0<\/em><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Thiazolidinediony (TZD) jsou \u0161iroce pou\u017e\u00edv\u00e1ny p\u0159i l\u00e9\u010db\u011b inzulinov\u00e9 resistence u pacient\u016f s&nbsp;diabetes mellitus 2.&nbsp;typu (DM). Tato l\u00e9\u010dba je v\u0161ak spojena s&nbsp;n\u011bkter\u00fdmi ne\u017e\u00e1douc\u00edmi \u00fa\u010dinky. Je proto zapot\u0159eb\u00ed nov\u00fdch l\u00e9\u010debn\u00fdch postup\u016f, kter\u00e9 by umo\u017enily pou\u017e\u00edt ni\u017e\u0161\u00edch (suboptim\u00e1ln\u00edch) d\u00e1vek TZD v&nbsp;kombinaci s&nbsp;dal\u0161\u00edmi farmakologick\u00fdmi \u010di dietetick\u00fdmi intervencemi. In our previous studies\u00a0(Kuda et al. 2009. Diabetologia;\u00a0Horakova et al. 2012. PLoS [&hellip;]<\/p>\n","protected":false},"author":1,"template":"","meta":{"_acf_changed":false,"inline_featured_image":false,"footnotes":""},"oddeleni":[149],"poskytovatel":[],"stav-projektu":[210],"class_list":["post-30122","vyzkumny-projekt","type-vyzkumny-projekt","status-publish","hentry","oddeleni-adipose-tissue-biology","stav-projektu-finished-projects"],"acf":[],"_links":{"self":[{"href":"https:\/\/fgu.cas.cz\/en\/wp-json\/wp\/v2\/vyzkumny-projekt\/30122","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/fgu.cas.cz\/en\/wp-json\/wp\/v2\/vyzkumny-projekt"}],"about":[{"href":"https:\/\/fgu.cas.cz\/en\/wp-json\/wp\/v2\/types\/vyzkumny-projekt"}],"author":[{"embeddable":true,"href":"https:\/\/fgu.cas.cz\/en\/wp-json\/wp\/v2\/users\/1"}],"version-history":[{"count":0,"href":"https:\/\/fgu.cas.cz\/en\/wp-json\/wp\/v2\/vyzkumny-projekt\/30122\/revisions"}],"wp:attachment":[{"href":"https:\/\/fgu.cas.cz\/en\/wp-json\/wp\/v2\/media?parent=30122"}],"wp:term":[{"taxonomy":"oddeleni","embeddable":true,"href":"https:\/\/fgu.cas.cz\/en\/wp-json\/wp\/v2\/oddeleni?post=30122"},{"taxonomy":"poskytovatel","embeddable":true,"href":"https:\/\/fgu.cas.cz\/en\/wp-json\/wp\/v2\/poskytovatel?post=30122"},{"taxonomy":"stav-projektu","embeddable":true,"href":"https:\/\/fgu.cas.cz\/en\/wp-json\/wp\/v2\/stav-projektu?post=30122"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}