{"id":32542,"date":"2024-09-20T09:00:56","date_gmt":"2024-09-20T07:00:56","guid":{"rendered":"https:\/\/fgu.cas.cz\/?post_type=vyzkumny-projekt&#038;p=32542"},"modified":"2024-11-12T09:17:24","modified_gmt":"2024-11-12T08:17:24","slug":"signaling-role-of-pancreatic-%ce%b2-cell-exosomes","status":"publish","type":"vyzkumny-projekt","link":"https:\/\/fgu.cas.cz\/en\/research-project\/signaling-role-of-pancreatic-%ce%b2-cell-exosomes\/","title":{"rendered":"Signaling role of pancreatic \u03b2-cell exosomes"},"content":{"rendered":"<p>This project investigates the signaling role and content of exosomes\u2014specialized extracellular vesicles\u2014produced by pancreatic beta cells. We are particularly focused on their signaling role in both normal physiology during glucose-induced insulin secretion and in pathological conditions linked to type 2 diabetes progression. We use pancreatic islets and a beta cell line as models in which we induce hyperglycemia by glucolipotoxic conditions mimicking the development of diabetes. Additionally, we will use beta cells where NADPH oxidase 4 is deleted. Its activity is important for efficient insulin efflux upon glucose stimulation (Plecita-Hlavata, L., et. al., 2020). In the case of its deficient function, insulin output is reduced, which is also one of the signs of advanced diabetes.\u00a0 Under these stress conditions, beta cells initiate signaling that involves exosomes, specifically through their miRNA and protein content. \u00a0This study will further explore how exosomes from stressed beta cells influence the signaling pathways in healthy beta cells, providing insights into cellular communication during the development of diabetes.<\/p>\n<p>Lydie Plecit\u00e1-Hlavat\u00e1,\u00a0Martin Jab\u016frek,\u00a0Blanka Holendov\u00e1,\u00a0Jan Tauber,\u00a0Vojt\u011bch Pavluch,\u00a0Zuzana Berkov\u00e1,\u00a0Monika Cahov\u00e1,\u00a0Katrin Schr\u00f6der,\u00a0Ralf P. Brandes,\u00a0Detlef Siemen,\u00a0Petr Je\u017eek; Glucose-Stimulated Insulin Secretion Fundamentally Requires H<sub>2<\/sub>O<sub>2<\/sub>\u00a0Signaling by NADPH Oxidase 4.\u00a0<em>Diabetes<\/em>\u00a01 July 2020; 69 (7): 1341\u20131354.\u00a0<a href=\"https:\/\/doi.org\/10.2337\/db19-1130\" target=\"_blank\" rel=\"noopener\">https:\/\/doi.org\/10.2337\/db19-1130<\/a><\/p>\n<p><img fetchpriority=\"high\" decoding=\"async\" class=\"alignnone  wp-image-36380\" src=\"https:\/\/fgu.cas.cz\/wp-content\/uploads\/2024\/09\/073-2-1.jpg\" alt=\"-\" width=\"341\" height=\"332\" title=\"\" srcset=\"https:\/\/fgu.cas.cz\/wp-content\/uploads\/2024\/09\/073-2-1.jpg 527w, https:\/\/fgu.cas.cz\/wp-content\/uploads\/2024\/09\/073-2-1-300x292.jpg 300w\" sizes=\"(max-width: 341px) 100vw, 341px\" \/>\u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 <img decoding=\"async\" class=\" wp-image-36382 alignnone\" src=\"https:\/\/fgu.cas.cz\/wp-content\/uploads\/2024\/09\/073-2-2.png\" alt=\"-\" width=\"310\" height=\"364\" title=\"\"><\/p>\n<p>Fig.1 Visualization of isolated exosomes produced by pancreatic beta cells by transmission electron microscopy and their size determination by nanoparticle tracking analysis.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>This project investigates the signaling role and content of exosomes\u2014specialized extracellular vesicles\u2014produced by pancreatic beta cells. We are particularly focused on their signaling role in both normal physiology during glucose-induced insulin secretion and in pathological conditions linked to type 2 diabetes progression. We use pancreatic islets and a beta cell line as models in which [&hellip;]<\/p>\n","protected":false},"author":5,"template":"","meta":{"_acf_changed":false,"inline_featured_image":false,"footnotes":""},"oddeleni":[186],"poskytovatel":[],"stav-projektu":[209],"class_list":["post-32542","vyzkumny-projekt","type-vyzkumny-projekt","status-publish","hentry","oddeleni-pancreatic-islet-research","stav-projektu-current-projects"],"acf":[],"_links":{"self":[{"href":"https:\/\/fgu.cas.cz\/en\/wp-json\/wp\/v2\/vyzkumny-projekt\/32542","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/fgu.cas.cz\/en\/wp-json\/wp\/v2\/vyzkumny-projekt"}],"about":[{"href":"https:\/\/fgu.cas.cz\/en\/wp-json\/wp\/v2\/types\/vyzkumny-projekt"}],"author":[{"embeddable":true,"href":"https:\/\/fgu.cas.cz\/en\/wp-json\/wp\/v2\/users\/5"}],"version-history":[{"count":0,"href":"https:\/\/fgu.cas.cz\/en\/wp-json\/wp\/v2\/vyzkumny-projekt\/32542\/revisions"}],"wp:attachment":[{"href":"https:\/\/fgu.cas.cz\/en\/wp-json\/wp\/v2\/media?parent=32542"}],"wp:term":[{"taxonomy":"oddeleni","embeddable":true,"href":"https:\/\/fgu.cas.cz\/en\/wp-json\/wp\/v2\/oddeleni?post=32542"},{"taxonomy":"poskytovatel","embeddable":true,"href":"https:\/\/fgu.cas.cz\/en\/wp-json\/wp\/v2\/poskytovatel?post=32542"},{"taxonomy":"stav-projektu","embeddable":true,"href":"https:\/\/fgu.cas.cz\/en\/wp-json\/wp\/v2\/stav-projektu?post=32542"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}