Přirozeně se vyskytující omega-3 PUFA s dlouhým řetězcem, jmenovitě kyselina eikosapentaenová (EPA; 20: 5 co-3), kyselina dokosapentaenová (DPA; 22: 5 co-3) a kyselina dokosahexaenová (DHA; 22: 6 co-3) vykazují více biologických účinků ...
Naturally occurring long-chain omega-3 PUFA, namely eicosapentaenoic acid (EPA; 20:5 ω-3), docosapentaenoic acid (DPA; 22:5 ω-3), and docosahexaenoic acid (DHA; 22:6 ω-3) exert multiple biological effects that are mediated either by these PUFA themselves (i.e. acting as PPARα ligands) or their bioactive metabolites. These bioactive compounds could be divided into several sub-families such as specialized pro-resolving mediators, epoxides, electrophilic oxo-derivatives, ethanolamines, acylglycerols, acylamides of amino acids or neurotransmitters, and FAHFAs, exerting mostly anti-inflammatory effects, also in adipose tissue .
► Ondrej Kuda, Martin Rossmeisl, Jan Kopecky✉
Omega-3 fatty acids and adipose tissue biology.
Mol Aspects Med. 2018 Dec;64:147-160 DOI https://doi.org/10.1016/j.mam.2018.01.004
This review provides evidence for the importance of white and brown adipose tissue (i.e. WAT and BAT) function for the maintenance of healthy metabolic phenotype and its preservation in response to omega-3 polyunsaturated fatty acids (omega-3 PUFA), namely in the context of diseased states linked to aberrant accumulation of body fat, systemic low-grade inflammation, dyslipidemia and insulin resistance. More specifically, the review deals with (i) the concept of immunometabolism, i.e. how adipose-resident immune cells and adipocytes affect each other and define the immune-metabolic interface; and (ii) the characteristic features of "healthy adipocytes" in WAT, which are relatively small fat cells endowed with a high capacity for mitochondrial oxidative phosphorylation, triacylglycerol/fatty acid (TAG/FA) cycling and de novo lipogenesis (DNL). The intrinsic metabolic features of WAT and their flexible regulations, reflecting the presence of "healthy adipocytes", provide beneficial local and systemic effects, including (i) protection against in situ endoplasmic reticulum stress and related inflammatory response during activation of adipocyte lipolysis; (ii) prevention of ectopic fat accumulation and dyslipidemia caused by increased hepatic VLDL synthesis, as well as prevention of lipotoxic damage of insulin signaling in extra-adipose tissues; and also (iii) increased synthesis of anti-inflammatory and insulin-sensitizing lipid mediators with pro-resolving properties, including the branched fatty acid esters of hydroxy fatty acids (FAHFAs), also depending on the activity of DNL in WAT. The "healthy adipocytes" phenotype can be induced in WAT of obese mice in response to various stimuli including dietary omega-3 PUFA, especially when combined with moderate calorie restriction, and possibly also with other life style (e.g. physical activity) or pharmacological (e.g. thiazolidinediones) interventions. While omega-3 PUFA could exert beneficial systemic effects by improving immunometabolism of WAT without a concomitant induction of BAT, it is currently not clear whether the metabolic effects of the combined intervention using omega-3 PUFA and calorie restriction or thiazolidinediones depend also on the activation of BAT function and/or the induction of brite/beige adipocytes in WAT. It remains to be established why omega-3 PUFA intervention in type 2 diabetic subjects does not improve insulin sensitivity and glucose homeostasis despite inducing various anti-inflammatory mediators in WAT, including the recently discovered docosahexaenoyl esters of hydroxy linoleic acid, the lipokines from the FAHFA family, as well as several endocannabinoid-related anti-inflammatory lipids. To answer the question whether and to which extent omega-3 PUFA supplementation could promote the formation of "healthy adipocytes" in WAT of human subjects, namely in the obese insulin-resistant patients, represents a challenging task that is of great importance for the treatment of some serious non-communicable diseases.
► Veronika Paluchova, Anders Vik, Tomas Cajka, Marie Brezinova, Kristyna Brejchova, Viktor Bugajev, Lubica Draberova, Petr Draber, Jana Buresova, Petra Kroupova, Kristina Bardova, Martin Rossmeisl, Jan Kopecky, Trond Vidar Hansen, Ondrej Kuda✉
Triacylglycerol-rich oils of marine origin are optimal nutrients for induction of polyunsaturated docosahexaenoic acid ester of hydroxy linoleic acid (13-DHAHLA) with anti-inflammatory properties in mice.
Molecular Nutrition and Food Research, in press DOI https://doi.org/10.1002/mnfr.201901238
Scope Docosahexaenoic acid ester of hydroxy linoleic acid (DHAHLA) is a bioactive lipids with anti‐inflammatory properties from the family of fatty acid esters of hydroxy fatty acids (FAHFA). Methods and results To explore the biosynthesis of 13‐DHAHLA from dietary oils, C57BL/6N mice were gavaged for 8 days with various corn oil / marine oil mixtures containing the same amount of DHA. Plasma levels of omega‐3 FAHFAs were influenced by the lipid composition of the mixtures but did not reflect the changes in bioavailability of polyunsaturated fatty acids in plasma. Triacylglycerol‐bound DHA and linoleic acid served as more effective precursors for 13‐DHAHLA synthesis than DHA bound in phospholipids or wax esters. Both 13(S)‐ and 13(R)‐DHAHLA inhibited antigen and PGE2‐induced chemotaxis and degranulation of mast cells to a comparable extent and 13(S)‐DHAHLA was identified as the predominant isomer in mouse adipose tissue. Conclusion Here we identified the optimal nutritional source of DHA, which supports production of anti‐inflammatory FAHFAs, as triacylglycerol‐based marine oil and also revealed a possible role of triacylglycerols in the synthesis of FAHFA lipokines.
- 13-DHAHLA is an anti-inflammatory lipid mediator.
- The authors investigate DHA-rich marine oils as potential nutritional sources of 13-DHAHLA precursors and explore anti-inflammatory properties of the bioactive lipid.
- The results suggest that triacylglycerol-based marine oils are superior to marine phospholipids and wax esters in the ability to increase levels of 13-DHAHLA in circulation.
- Both 13(S)- and 13(R)-DHAHLA inhibited antigen and PGE2-induced chemotaxis and degranulation of mast cells.
One of the DHA metabolites – polyunsaturated FAHFA 13-DHAHLA exerts anti-inflammatory effects of macrophages and mast cells [2, 3]. DHA-rich triacylglycerol-based marine oils are superior to marine phospholipids and wax esters in the ability to increase levels of 13-DHAHLA in circulation. Interestingly, the chemical form of the oil (lipid class) was more important that the fatty acid composition.
► Martin Rossmeisl✉, Jana Pavlisova, Kristina Bardova, Veronika Kalendova, Jana Buresova, Ondrej Kuda, Petra Kroupova, Barbora Stankova, Eva Tvrzicka, Eva Fiserova, Olga Horakova, Jan Kopecky
Increased plasma levels of palmitoleic acid may contribute to beneficial effects of Krill oil on glucose homeostasis in dietary obese mice
Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, May 2020, 158732, In Press DOI https://doi.org/10.1016/j.bbalip.2020.158732
Omega-3 polyunsatuarted fatty acids (PUFA) are associated with hypolipidemic and anti-inflammatory effects. However, omega-3 PUFA, usually administered as triacylglycerols or ethyl esters, could also compromise glucose metabolism, especially in obese type 2 diabetics.
- Omega-3 PUFA administered as a triacylglycerol concentrate or Krill oil phospholipids.
- Omega-3 phospholipids preserved insulin sensitivity in mice fed a high-fat diet.
- Their insulin-sensitizing effect was better than that of omega-3 triacylglycerols.
- Krill oil effectively increased adiponectin and reduced endocannabinoid plasma levels.
- Palmitoleic acid from Krill oil may help improve glucose homeostasis.
► Marina Oseeva, Veronika Paluchova, Petr Zacek, Petra Janovska, Tomas Mracek, Martin Rossmeisl, Dana Hamplova, Nadezda Cadova, Iva Stohanzlova, Pavel Flachs, Jan Kopecky✉, OndrejKuda✉
Omega-3 index in the Czech Republic: No difference between urban and rural populations
Chem Phys Lipids. 2019 May;220:23-27 DOI https://doi.org/10.1016/j.chemphyslip.2019.02.006
Naturally occurring long-chain omega-3 PUFA such as eicosapentaenoic acid (EPA; 20:5 ω-3) and docosahexaenoic acid (DHA; 22:6 ω-3) exert multiple effects on health, which are related to the intake of these lipids in the diet and correlate with the levels of omega-3 PUFA in the body. These levels are reflected by the omega-3 PUFA index, i.e. the EPA and DHA content as % of all fatty acids in red blood cells. The aim of this study was to evaluate omega-3 index in the Czech Republic, using blood samples collected from the capital city (n = 476) and the rural region (n = 388). The mean omega-3 index was 3.56 mol % with a maximal value of 8.10% and a minimal value of 1.12%. There was no difference in the index value between rural and urban / industrial regions, but this value was higher in subjects who reported eating fish or omega-3 PUFA supplements. In conclusion, the results indicated suboptimal values of the omega-3 index in the Czech population independent of the sampling region.
We have explored the omega-3 index in Czech population within a project QUALITAS - Wellbeing in health and disease (2015-2021). No difference between rural and urban population was observed, but low omega-3 index suggested that the consumption of marine fish or other omega-3 PUFA sources should be increased .
► Viktor Bugajev, Tomas Paulenda, Pavol Utekal, Michal Mrkacek, Ivana Halova, Ladislav Kuchar, Ondrej Kuda, Petra Vavrova, Björn Schuster, Sergio Fuentes-Liso, Lucie Potuckova, Daniel Smrz, Sara Cernohouzova, Lubica Draberova, Monika Bambouskova, Petr Draber
Crosstalk between ORMDL3, serine palmitoyltransferase, and 5-lipoxygenase in the sphingolipid and eicosanoid metabolic pathways
J Lipid Res Volume 62, 2021, 100121 DOI https://doi.org/10.1016/j.jlr.2021.100121
Leukotrienes (LTs) and sphingolipids are critical lipid mediators participating in numerous cellular signal transduction events and developing various disorders, such as bronchial hyperactivity leading to asthma. Enzymatic reactions initiating production of these lipid mediators involve 5-lipoxygenase (5-LO)-mediated conversion of arachidonic acid to LTs and serine palmitoyltransferase (SPT)-mediated de novo synthesis of sphingolipids. Previous studies have shown that endoplasmic reticulum membrane protein ORM1-like protein 3 (ORMDL3) inhibits the activity of SPT and subsequent sphingolipid synthesis. However, the role of ORMDL3 in the synthesis of LTs is not known. In this study, we used peritoneal-derived mast cells isolated from ORMDL3 KO or control mice and examined their calcium mobilization, degranulation, NF-κB inhibitor-α phosphorylation, and TNF-α production. We found that peritoneal-derived mast cells with ORMDL3 KO exhibited increased responsiveness to antigen. Detailed lipid analysis showed that compared with WT cells, ORMDL3-deficient cells exhibited not only enhanced production of sphingolipids but also of LT signaling mediators LTB4, 6t-LTB4, LTC4, LTB5, and 6t-LTB5. The crosstalk between ORMDL3 and 5-LO metabolic pathways was supported by the finding that endogenous ORMDL3 and 5-LO are localized in similar endoplasmic reticulum domains in human mast cells and that ORMDL3 physically interacts with 5-LO. Further experiments showed that 5-LO also interacts with the long-chain 1 and long-chain 2 subunits of SPT. In agreement with these findings, 5-LO knockdown increased ceramide levels, and silencing of SPTLC1 decreased arachidonic acid metabolism to LTs to levels observed upon 5-LO knockdown. These results demonstrate functional crosstalk between the LT and sphingolipid metabolic pathways, leading to the production of lipid signaling mediators.
 O. Kuda, M. Rossmeisl, J. Kopecky, Omega-3 fatty acids and adipose tissue biology, Mol. Aspects Med. 64(4) (2018) 147-160.
 O. Kuda, M. Brezinova, M. Rombaldova, B. Slavikova, M. Posta, P. Beier, P. Janovska, J. Veleba, J. Kopecky, Jr., E. Kudova, T. Pelikanova, J. Kopecky, Docosahexaenoic Acid-Derived Fatty Acid Esters of Hydroxy Fatty Acids (FAHFAs) With Anti-inflammatory Properties, Diabetes 65(9) (2016) 2580-90.
 V. Paluchova, Triacylglycerol-rich oils of marine origin are optimal nutrients for induction of polyunsaturated docosahexaenoic acid ester of hydroxy linoleic acid (13-DHAHLA) with anti-inflammatory properties in mice, Mol. Nutr. Food Res. (2020).
 M. Oseeva, V. Paluchova, P. Zacek, P. Janovska, T. Mracek, M. Rossmeisl, D. Hamplova, N. Cadova, I. Stohanzlova, P. Flachs, J. Kopecky, O. Kuda, Omega-3 index in the Czech Republic: No difference between urban and rural populations, Chem Phys Lipids 220 (2019) 23-27.