Laboratory of Molecular Neurobiology

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Left: GFP-labeled hippocampal granule cells (green), in utero electroporated. Middle: Dendrites (arrows) and dendritic spines (inset) of cortical pyramidal neurons (red) and their 3D model. Right: NFL antibody-stained peripheral neurons in E11.5 embryos.

About the Laboratory

Defects in signaling cascades are considered to contribute to many neurodevelopmental and neurodegenerative disorders, but the exact molecular mechanisms underlying the onset of these diseases are so far largely unknown. Using mouse models and primary neuron cultures, we focus on identification of genes, molecular mechanism and signaling cascades that regulate neuron polarization, axonal/dendritic growth and guidance in the developing nervous system, and their deregulation that leads to neurodevelopmental disorders as autism spectrum disorders, epilepsy or schizophrenia.

Our work focuses on:

the function of microtubule associated proteins of CRMP family and their isoforms in neural development
regulation of CRMP isoforms by prolyl isomerases during growth and guidance of axons/dendrites
the efect of tubulin pottranslational modifications and microtubule associated proteins on microtubule dynamics and cortical development in vitro and in vivo

Projects

Conformational stress in Alzheimer’s disease

Molecular regulation of axon growth and guidance

Publications

Procházková; N. - Nguyenova; M.-T. - Řehořová; M. - Kudláček; J. - Chvojka; J. - Žiak; Jakub - Balaštík; Martin - Otáhal; J. - Jiruška; P. - Novák; O. NeuroPorator: An open-source; current-limited electroporator for safe in utero gene transfer. Journal of Neuroscience Methods. 2024; 406(June); 110126.

IF = 2.7

Chvojka; J. - Procházková; N. - Řehořová; M. - Kudláček; J. - Kylarová; S. - Králiková; M. - Buran; Peter - Weissová; Romana - Balaštík; Martin - Jefferys; J. G. R. - Novák; O. - Jiruška; P. Mouse model of focal cortical dysplasia type II generates a wide spectrum of high-frequency activities. Neurobiology of Disease. 2024; 190(January); 106383.

IF = 6.1

Obšilová; Veronika - Obšil; T. The yeast 14-3-3 proteins Bmh1 and Bmh2 regulate key signaling pathways. Frontiers in molecular biosciences. 2024; 11(Jan 24); 1327014.

IF = 5.0

Druga; Rastislav - Mareš; Pavel - Salaj; M. - Kubová; Hana Degenerative Changes in the Claustrum and Endopiriform Nucleus after Early-Life Status Epilepticus in Rats. International Journal of Molecular Sciences. 2024; 25(2); 1296.

IF = 5.6

Cheema; Marie Munawar - Kotrbová Macáková; Zuzana - Hrčka Krausová; Barbora - Adla; Santosh Kumar - Slavíková; Barbora - Chodounská; Hana - Kratochvíl; Miroslav - Vondrášek; Jiří - Sedlák; David - Balaštík; Martin - Kudová; Eva 5?-reduced neuroactive steroids as modulators of growth and viability of postnatal neurons and glia. Journal of Steroid Biochemistry and Molecular Biology. 2024; 239(May); 106464.

IF = 4.1

Abramová; Vera - Leal Alvarado; Vanessa - Hill; M. - Smejkalová; Tereza - Malý; Michal - Valeš; Karel - Dittert; Ivan - Božíková; Paulina - Kysilov; Bohdan - Hrčka Krausová; Barbora - Vyklický; Vojtěch - Balík; Aleš - Fili; Klevinda - Kořínek; Miloslav - Chodounská; Hana - Kudová; Eva - Číž; D. - Martinovič; J. - Černý; Jiří - Bartůněk; Petr - Vyklický ml.; Ladislav Effects of Pregnanolone Glutamate and Its Metabolites on GABAA and NMDA Receptors and Zebrafish Behavior. ACS Chemical Neuroscience. 2023; 14(10); 1870-1883.

IF = 5.0