The instrument is dedicated to metabolomics / lipidomics (small molecules).
Specs
– LC-MS/MS system
– mass spectrometer QTRAP 5500 from AB SCIEX
– liquid chromatography Ultimate 3000 RSLC from Dionex/Thermo
– ion source Turbo V (ESI a APCI)
– scans: Q1 MS, Q3 MS, Product Ion, Precursor Ion, Neutral Loss or Gain, MRM, EMS, EPI, ER, MS3, MRM3, TripleTrap
Methods
– Kvantitative analysis of oxylipins – eicosanoids, protectins, resolvins, oxidized fatty acids
– Kvantitative analysis of cholesteryl esters – free cholesterol + C2 – C26:x esters
– Kvantitative analysis of steroids
– Kvantitative analysis of acylcarnitines and amino acids (FIA or HILIC, kit Chromsystems)
– Kvantitative analysis of drugs in plasma (metformin, glitazones,…)
– Kvantitative analysis of glycerophospholipids (shotgun or LC-MS/MS) – PC, PE, PS, PG, PI, lyso*…
– Kvantitative analysis of endocannabinoids – anandamide, 2-arachidonoyl glycerol,…
– Kvalitative analysis of triacylglycerols
Contact: Ondrej Kuda, Ph.D., tel +420 296 443 707 office, +420 296 442 816 lab QTRAP, email: ondrej.kuda@fgu.cas.cz
