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Laboratory of Developmental Epileptology

Laboratory of Developmental Epileptology

The research at our Laboratory focuses on the pathophysiology of epilepsy and epileptic seizures in adulthood and particularly in the developing brain. In our research we are utilizing modern electrophysiological, imaging, biochemical and pharmacological techniques. To elucidate the cellular and network mechanisms involved in epilepsy and to increase the translational potential of new observations we are using extensively chronic models of acquired epilepsy and also genetic models and also collaborate with leading epilepsy centres.

Our primary research topics are:

  • Consequences of brain injury during early stages of the brain development
  • Mechanisms responsible for seizure initiation and termination.
  • Impact of seizures on the developing brain
  • Development of new diagnostic techniques of epilepsy
  • Cognitive and behavioural consequences of epilepsy
  • The role of oxidative stress in pathogenesis of epilepsy and seizures during the development
  • Impact of antiepileptic therapy of the brain development
  • Age-specific mechanisms of seizure initiation and propagation

Projects

Consequences of early brain-damage and prevention of development of epilepsy and functional damage

Brain-damaging insult triggers a cascade of molecular, cellular and structural changes that eventually lead to behavioral alterations and development of epilepsy. The response to the particular brain insult is highly age-dependent. Our aim is to study effects of early insults on the brain development at the molecular, cellular, and circuitry levels (maturation of receptors or ionic channel structures, vascularisation, myelination, circuitry maturation, etc.) and consequently on maturation of more complex brain functions (cognitive functions, sensorimotor skills, social and emotional behavior, brain excitability, etc.). Results of our research can be used to develop age specific pharmacological strategies for prevention of epilepsy development and disturbances of brain function.   More

Achievements

Publications

Uttl; Libor - Hložek; T. - Mareš; Pavel - Páleníček; T. - Kubová; Hana . Anticonvulsive Effects and Pharmacokinetic Profile of Cannabidiol (CBD) in the Pentylenetetrazol (PTZ) or N-Methyl-D-Aspartate (NMDA) Models of Seizures in Infantile Rats . International Journal of Molecular Sciences. 2022; 23(1)); 94 . IF = 6.208 [ASEP] [ doi ]
Kudová; Eva - Mareš; Pavel - Hill; M. - Vondráková; Kateřina - Tsenov; Grygoriy - Chodounská; Hana - Kubová; Hana - Valeš; Karel . The Neuroactive Steroid Pregnanolone Glutamate: Anticonvulsant Effect; Metabolites and Its Effect on Neurosteroid Levels in Developing Rat Brains . Pharmaceuticals. 2022; 15(1)); 49 . IF = 5.215 [ASEP] [ doi ]
Fábera; Petr - Uttl; Libor - Kubová; Hana - Tsenov; Grygoriy - Mareš; Pavel . Adenosine Kinase Isoforms in the Developing Rat Hippocampus after LiCl/Pilocarpine Status Epilepticus . International Journal of Molecular Sciences. 2022; 23(5)); 2510 . IF = 6.208 [ASEP] [ doi ]
Daněk; Jan - Danačíková; Šárka - Kala; David - Svoboda; Jan - Kapoor; Sonam - Pošusta; Antonín - Folbergrová; Jaroslava - Tauchmannová; Kateřina - Mráček; Tomáš - Otáhal; Jakub . Sulforaphane Ameliorates Metabolic Changes Associated With Status Epilepticus in Immature Rats . Frontiers in Cellular Neuroscience. 2022; 16(Mar 15)); 855161 . IF = 6.147 [ASEP] [ doi ]
Bencúrová; P. - Laakso; H. - Salo; R. A. - Paasonen; E. - Manninen; E. - Paasonen; J. - Michaeli; S. - Mangia; S. - Bareš; M. - Brázdil; M. - Kubová; Hana - Gröhn; O. Infantile status epilepticus disrupts myelin development . Neurobiology of Disease. 2022; 162(Jan)); 105566 . IF = 7.046 [ASEP] [ doi ]
Žarković; D. - Šorfová; M. - Tufano; J. J. - Kutílek; P. - Vítečková; S. - Ravnik; D. - Groleger-Sršen; K. - Cikajlo; I. - Otáhal; Jakub . Gait changes following robot-assisted gait training in children with cerebral palsy . Physiological Research. 2021; 70(Suppl.3); S397-S408 . IF = 2.139 [ASEP] [ doi ]
Salaj; M. - Barinka; F. - Kubová; Hana - Druga; Rastislav . Differences in Expression of Calcium Binding Proteins in the Perirhinal and Retrosplenial Cortex of the Rat . Physiological Research. 2021; 70(2); 273-285 . IF = 2.139 [ASEP] [ doi ]
Petrásek; T. - Vojtěchová; I. - Klovrza; O. - Tučková; K. - Vejmola; Č. - Rak; J. - Šuláková; A. - Kaping; D. - Bernhardt; N. - de Vries; P. J. - Otáhal; Jakub - Waltereit; R. mTOR inhibitor improves autistic-like behaviors related to Tsc2 haploinsufficiency but not following developmental status epilepticus . Journal of Neurodevelopmental Disorders. 2021; 13(1)); 14 . IF = 4.074 [ASEP] [ doi ]
Mareš; Pavel - Tsenov; Grygoriy - Kubová; Hana . Anticonvulsant Action of GluN2A-Preferring Antagonist PEAQX in Developing Rats . Pharmaceutics. 2021; 13(3)); 415 . IF = 6.525 [ASEP] [ doi ]
Mareš; Pavel - Kubová; Hana . Perampanel exhibits anticonvulsant action against pentylentetrazol-induced seizures in immature rats . Epilepsy Research. 2021; 169(Jan)); 106523 . IF = 2.991 [ASEP] [ doi ]
Mareš; Pavel - Kozlová; Lucie - Mikulecká; Anna - Kubová; Hana . The GluN2B-Selective Antagonist Ro 25-6981 Is Effective against PTZ-Induced Seizures and Safe for Further Development in Infantile Rats . Pharmaceutics. 2021; 13(9)); 1482 . IF = 6.525 [ASEP] [ doi ]
Kudláček; Jan - Chvojka; Jan - Kumpošt; Vojtěch - Heřmanovská; Barbora - Pošusta; Antonín - Jefferys; J. G. R. - Maturana; M. I. - Novák; O. - Cook; M. J. - Otáhal; Jakub - Hlinka; Jaroslav - Jiruška; Přemysl . Long-term seizure dynamics are determined by the nature of seizures and the mutual interactions between them . Neurobiology of Disease. 2021; 154(July)); 105347 . IF = 7.046 [ASEP] [ doi ]
Janča; R. - Jahodová; A. - Hlinka; Jaroslav - Ježdík; P. - Svobodová; L. - Kudr; M. - Kalina; A. - Marusič; P. - Kršek; P. - Jiruška; Přemysl . Ictal Gamma-Band Interactions Localize Ictogenic Nodes of the Epileptic Network in Focal Cortical Dysplasia . Clinical Neurophysiology. 2021; 132(8); 1927-1936 . IF = 4.861 [ASEP] [ doi ]
Chvojková; Markéta - Kubová; Hana - Valeš; Karel . Effects of Dizocilpine; Midazolam and Their Co-Application on the Trimethyltin (TMT)-Induced Rat Model of Cognitive Deficit . Brain Sciences. 2021; 11(3)); 400 . IF = 3.333 [ASEP] [ doi ]
Chvojka; Jan - Kudláček; Jan - Chang; W.-Ch. - Novák; O. - Tomáška; F. - Otáhal; Jakub - Jefferys; J. G. R. - Jiruška; Přemysl . The role of interictal discharges in ictogenesis-A dynamical perspective . Epilepsy and Behavior. 2021; 121(Part B Aug)); 106591 . IF = 3.337 [ASEP] [ doi ]
Folbergrová; Jaroslava - Ješina; Pavel - Otáhal; Jakub . Treatment With Resveratrol Ameliorates Mitochondrial Dysfunction During the Acute Phase of Status Epilepticus in Immature Rats . Frontiers in Neuroscience. 2021; 15(Mar 5)); 634378 . IF = 5.152 [ASEP] [ doi ]
Bencúrová; P. - Baloun; J. - Hynšt; J. - Oppelt; J. - Kubová; Hana - Pospíšilová; Š. - Brázdil; M. Dynamic miRNA changes during the process of epileptogenesis in an infantile and adult-onset model . Scientific Reports. 2021; 11(1)); 9649 . IF = 4.996 [ASEP] [ doi ]
Vondráková; Kateřina - Novotný; P. - Kubová; Hana - Pošusta; Antonín - Boron; J. - Fáberová; Veronika - Fabene; P. F. - Burchfiel; J. - Tsenov; Grygoriy . Electrographic seizures induced by activation of ETA and ETB receptors following intrahippocampal infusion of endothelin-1 in immature rats occur by different mechanisms . Experimental Neurology. 2020; 328(Jun)); 113255 . IF = 5.330 [ASEP] [ doi ]
Vodička; Martin - Vavřínová; Anna - Mikulecká; Anna - Zicha; Josef - Behuliak; Michal . Hyper-reactivity of HPA axis in Fischer 344 rats is associated with impaired cardiovascular and behavioral adaptation to repeated restraint stress . STRESS-THE INTERNATIONAL JOURNAL ON THE BIOLOGY OF STRESS. 2020; 23(6); 667-677 . IF = 3.493 [ASEP] [ doi ]
Riljak; V. - Laštůvka; Z. - Mysliveček; J. - Borbélyová; V. - Otáhal; Jakub . Early Postnatal Hypoxia Induces Behavioral Deficits but not Morphological Damage in the Hippocampus in Adolescent Rats . Physiological Research. 2020; 69(1); 165-179 . IF = 1.881 [ASEP] [ doi ]
Maturana; M. I. - Meisel; Ch. - Dell; K. - Karoly; P. J. - D´Souza; W. - Grayden; D. B. - Burkitt; A. N. - Jiruška; Přemysl - Kudláček; Jan - Hlinka; Jaroslav - Cook; M. J. - Kuhlmann; L. - Freestone; D. R. Critical slowing down as a biomarker for seizure susceptibility . Nature Communications. 2020; 11(1)); 2172 . IF = 14.919 [ASEP] [ doi ]
Mareš; Pavel - Kudová; Eva - Valeš; Karel - Kubová; Hana . Three neurosteroids as well as GABAergic drugs do not convert immediate postictal potentiation to depression in immature rats . Pharmacological Reports. 2020; 72(6); 1573-1578 . IF = 3.024 [ASEP] [ doi ]
Mareš; Pavel - Kubová; Hana . Interaction of GABA(A) and GABA(B) antagonists after status epilepticus in immature rats . Epilepsy and Behavior. 2020; 102(Jan)); 106683 . IF = 2.937 [ASEP] [ doi ]
Laštůvka; Z. - Borbélyová; V. - Janišová; K. - Otáhal; Jakub - Mysliveček; J. - Riljak; V. Neonatal Hypoxic-Ischemic Brain Injury Leads to Sex-Specific Deficits in Rearing and Climbing in Adult Mice . Physiological Research. 2020; 69(Suppl.3); S499-S512 . IF = 1.881 [ASEP] [ doi ]
Kubová; Hana - Bendová; Z. - Moravcová; S. - Pačesová; D. - Rocha; L. - Mareš; Pavel . Neonatal Clonazepam Administration Induced Long-Lasting Changes in GABA(A) and GABA(B) Receptors . International Journal of Molecular Sciences. 2020; 21(9)); 3184 . IF = 5.923 [ASEP] [ doi ]

People

Doc. PharmDr. Hana Kubová, DrSc.
Head of the Laboratory
Prof. MUDr. Jakub Otáhal, Ph.D.
Deputy Head of the Laboratory
RNDr. Jaroslava Folbergrová, DrSc.
Senior Researcher
Prof. MUDr. Přemysl Jiruška, Ph.D.
Senior Researcher
Prof. MUDr. Pavel Mareš, DrSc.
Senior Researcher
RNDr. Nina Mikulecká, Ph.D.
Senior Researcher
Mgr. Grygoriy Tsenov, Ph.D.
Senior Researcher
Ing. Jan Kudláček​, Ph.D.
Postdoctoral Fellow
Ing. Antonín Pošusta, Ph.D.​ 
Postdoctoral Fellow
  Mgr. Šárka Danačíková
PhD Student
  Mgr. Jan Chvojka
PhD Student
 

Mgr. David Kala 

PhD Student

  RNDr. Jan Svoboda, Ph.D.
PhD Student
  Mgr. Zina Ben Salemová
PhD Student
  Blanka Čejková​​ 
Technician
 

Martin Kučera

Research Assistant

 

RNDr. Šárka Růžičková, Ph.D.

Research Assistant

Eva Lažková​ 
Technician
Irina Necheva​​ 
Technician
  Helena Havelková
Technician
Ivana Rabasová​​​ 
Personal Assistant