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Laboratory of Membrane Transport

Laboratory of Membrane Transport Sodium and potassium transporting protein observed in yeast cells through a fluorescence microscope. Green fluorescence corresponds to the protein localized in the cell plasma membrane; red fluorescence visualizes membranes of vacuoles.

We study the proteins transporting compounds and signals across the cell membranes. These proteins, called transporters, assure the uptake of nutrients into the cells, efflux of waste compounds from cells and communication with the environment.  To study the animal and plant transporters, we mainly use a model eukaryotic microorganism – yeasts. Our research is focused mainly on:

  • Structure, function and regulation of cell transport systems at protein molecule level.
  • Role of transporters in specific cell properties and in diseases.
  • Role of transporters in Candida virulence and pathogenicity.
  • Development of new techniques to estimate cell physiological parameters.

Projects

Cell cation and pH homeostasis

The intracellular concentration of potassium and sodium cations, as well as of protons (pH), is strictly regulated via the activity of a series of membrane proteins that mediate the flux of cations and protons with various transport mechanisms. Incorrect functioning of some transporters results in serious disorders and diseases. We study in detail the roles of individual transporters and the impact of their activity on cell fitness. More

Transporters reflected in specific properties of nonconventional yeasts

Some, so-called non-conventional, yeast species survive extreme changes in the environmental pH, temperature, or osmotic pressure. We aim to identify and characterize specific transporters whose activity contributes to the ability to survive adverse environmental conditions. The acquired knowledge will help to improve the properties of yeast species used in industrial processes. More

Achievements

Participation in project "Otevřená věda"

Our laboratory is already for many years involved in project Otevřená věda organised by Czech Academy of Sciences. The mission of this successful project is to lure enthusiastic students from secondary schools and show them how amusing the science can be. More

Publications

Papoušková; Klára - Gómez; M. - Kodedová; Marie - Ramos; J. - Zimmermannová; Olga - Sychrová; Hana . Heterologous expression reveals unique properties of Trk K+ importers from nonconventional biotechnologically relevant yeast species together with their potential to support Saccharomyces cerevisiae growth . Yeast. 2023; 40(2); 68-83 . IF = 2.6 [ASEP] [ doi ]
Masaryk; Jakub - Kale; Deepika - Pohl; Pavel - Ruiz-Castilla; F. J. - Zimmermannová; Olga - Obšilová; Veronika - Ramos; J. - Sychrová; Hana . The second intracellular loop of the yeast Trk1 potassium transporter is involved in regulation of activity; and interaction with 14–3-3 proteins . Computational and Structural Biotechnology Journal. 2023; 21(April); 2705-2716 . IF = 6.0 [ASEP] [ doi ]
Kodedová; Marie - Liška; V. - Mosinger; J. - Sychrová; Hana . Light-induced antifungal activity of nanoparticles with an encapsulated porphyrin photosensitizer . Microbiological Research. 2023; 269(April)); 127303 . IF = 6.7 [ASEP] [ doi ]
Brockhurst; M. - Cavet; J. - Diggle; S. P. - Grainger; D. - Mangenelli; R. - Sychrová; Hana - Martin-Verstraete; I. - Welch; M. - Palmer; T. - Thomas; G.H. Shaping microbiology for 75 years: highlights of research published in Microbiology. Part 1 - Physiology and growth . Microbiology. 2023; 169(6); 001356 . IF = 2.8 [ASEP] [ doi ]
Brockhurst; M. - Cavet; J. - Diggle; S. P. - Grainger; D. - Mangenelli; R. - Sychrová; Hana - Martin-Verstraete; I. - Welch; M. - Palmer; T. - Thomas; G.H. Shaping microbiology for 75 years: highlights of research published in Microbiology. Part 2 - Communities and evolution . Microbiology. 2023; 169(6); 001357 . IF = 2.8 [ASEP] [ doi ]
Antunes; M. - Kale; Deepika - Sychrová; Hana - Sá-Correia; I. The Hrk1 kinase is a determinant of acetic acid tolerance in yeast by modulating H+ and K+ homeostasis . Microbial cell. 2023; 10(12); 261-276 . IF = 4.6 [ASEP] [ doi ]
Velázquez; Diego - Průša; Vojtěch - Masrati; G. - Yariv; E. - Sychrová; Hana - Ben-Tal; N. - Zimmermannová; Olga . Allosteric links between the hydrophilic N-terminus and transmembrane core of human Na+/H+ antiporter NHA2 . Protein Science. 2022; 31(12)); e4460 . IF = 8.0 [ASEP] [ doi ]
Masaryk; Jakub - Sychrová; Hana . Yeast Trk1 Potassium Transporter Gradually Changes Its Affinity in Response to Both External and Internal Signals . Journal of Fungi. 2022; 8(5)); 432 . IF = 4.7 [ASEP] [ doi ]
Mane; S. - Pathan; E. - Tupe; S. - Deshmukh; S. - Kale; Deepika - Ghormade; V. - Chaudhari; B. - Deshpande; M. Isolation and Characterization of Chitosans from Different Fungi with Special Emphasis on Zygomycetous Dimorphic Fungus Benjaminiella poitrasii: Evaluation of Its Chitosan Nanoparticles for the Inhibition of Human Pathogenic Fungi . Biomacromolecules. 2022; 23(3); 808-815 . IF = 6.2 [ASEP] [ doi ]
Zimmermannová; Olga - Felcmanová; Kristina - Sácká; Lenka - Colinet; A. S. - Morsomme; P. - Sychrová; Hana . K+-specific importers Trk1 and Trk2 play different roles in Ca2+ homeostasis and signalling in Saccharomyces cerevisiae cells . FEMS Yeast Research. 2021; 21(3)); foab015 . IF = 2.923 [ASEP] [ doi ]
Ruiz-Castilla; F. J. - Bieber; Jan - Caro; G. - Michán; C. - Sychrová; Hana - Ramos; J. Regulation and activity of CaTrk1; CaAcu1 and CaHak1; the three plasma membrane potassium transporters in Candida albicans . Biochimica Et Biophysica Acta-Biomembranes. 2021; 1863(1)); 183486 . IF = 4.019 [ASEP] [ doi ]
Papoušková; Klára - Moravcová; Michaela - Masrati; G. - Ben-Tal; N. - Sychrová; Hana - Zimmermannová; Olga . C5 conserved region of hydrophilic C-terminal part of Saccharomyces cerevisiae Nha1 antiporter determines its requirement of Erv14 COPII cargo receptor for plasma-membrane targeting . Molecular Microbiology. 2021; 115(1); 41-57 . IF = 3.979 [ASEP] [ doi ]
Dušková; Michala - Cmunt; Denis - Papoušková; Klára - Masaryk; Jakub - Sychrová; Hana . Minority potassium-uptake system Trk2 has a crucial role in yeast survival of glucose-induced cell death . Microbiology. 2021; 167(6)); 001065 . IF = 2.956 [ASEP] [ doi ]
Dauss; E. - Papoušková; Klára - Sychrová; Hana - Rapoport; A. Anhydrobiosis in yeast: role of cortical endoplasmic reticulum protein Ist2 in Saccharomyces cerevisiae cells during dehydration and subsequent rehydration . Antonie van Leeuwenhoek International Journal of General and Molecular Microbiology. 2021; 114(7); 1069-1077 . IF = 2.158 [ASEP] [ doi ]
Albacar; M. - Sácká; Lenka - Calafí; C. - Velázquez; Diego - Casamayor; A. - Ariňo; J. - Zimmermannová; Olga . The Toxic Effects of Ppz1 Overexpression Involve Nha1-Mediated Deregulation of K+ and H+ Homeostasis . Journal of Fungi. 2021; 7(12)); 1010 . IF = 5.724 [ASEP] [ doi ]

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People

Hana Sychrová, DrSc.
Head of the Laboratory
Olga Zimmermannová, Ph.D. (née Kinclová)
Deputy Head of the Laboratory
  Marie Kodedová, Ph.D.
Postdoctoral Fellow
Klára Papoušková, Ph.D. (née Velková)
Postdoctoral Fellow

Michala Dušková, Ph.D. (née Bubnová)
Postdoctoral Fellow

 

Bc. Kristina Knopfová

pregraduální student

 

Viktorie Stojková

Undergraduate Student

Viktorie Radová

Undergraduate Student

Karolína Černá

Undergraduate Student

Pavla Herynková
Technician