Metabolism of bioactive lipids
The Laboratory focuses on the analysis of lipid mediators (eicosanoids, docosanoids, endocannabinoids) using the UPLC-MS/MS method, in particular to identify the source of production of various mediators and their effect on metabolism and immune cells (i.e. adipocytes vs. macrophages). Currently, the lab is exploring new anti-diabetic lipids, fatty acid esters of hydroxy fatty acids and lipid-related metabolic pathways using stable isotopes and LIMeX platform.
GTTAtlas - interactive metabolomics and lipidomics atlas of mouse tissues during oral glucose tolerance test (OGTT).
MetaboAtlas21 - a comprehensive atlas of the mouse metabolome and lipidome.
LORA - a Lipid Over-Representation Analysis tool.
Projects
Software:
GTTAtlas
MetaboAtlas21
Mapper
Reaction network viewer
LORA
Methodological highlighs from publications using small molecule omics techniques.
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White adipose tissue (WAT) is a complex endocrine organ and its low-grade inflammation in obesity contributes to the development of metabolic disorders. In 2014, a class of WAT-born lipid mediators - fatty acid esters of hydroxy fatty acids (FAHFA) was discovered. FAHFAs are endogenous lipids with anti-inflammatory and anti-diabetic properties, including the enhancement of glucose tolerance, and insulin and glucagon-like peptide 1 (GLP-1) secretion while reducing inflammatory responses [1-5].
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Naturally occurring long-chain omega-3 PUFA, namely eicosapentaenoic acid (EPA; 20:5 ω-3), docosapentaenoic acid (DPA; 22:5 ω-3), and docosahexaenoic acid (DHA; 22:6 ω-3) exert multiple biological effects...
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Our lab participates in OPEN SCIENCE initiative and hosts high school students with their project in metabolomics.
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We run local instances of several services focused on Metabolomics, Lipidomics, Statistical analysis and Chemometrics. As the hardware resources are limited, these tools are available only within campus LAN.
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MetaboAnalyst is a great tool for analysis of metabolomics data. Here are some tips how to install MetaboAnalyst locally on a linux workstation. Such installation could be tailored to meet individual requirements (default setting, fonts, etc.).
Starting point is a runnig installation of Ubuntu 18.04.3 LTS.
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CRediT (Contributor Roles Taxonomy) is high-level taxonomy, including 14 roles, that can be used to represent the roles typically played by contributors to scientific scholarly output. The roles describe each contributor’s specific contribution to the scholarly output. This web tool helps to summarize contributions of individual authors and prepare the Author contributions paragraph for a scientific journal.
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The main objective of this project is to explore structure, mechanisms of action, anti-inflammatory and anti-diabetic effects of novel lipokines – branched fatty acid esters of hydroxy fatty acids derived from polyunsaturated fatty acids of n-3 series (Omega-3) using obesity and inflammation models.
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Obesity is associated with an increased risk of developing metabolic syndrome, type 2 diabetes and cardiovascular disease. The adipose tissue significantly affects the whole-body metabolism of carbohydrates and lipids, which also involves fatty acid esters of hydroxy fatty acids (FAHFA), lipid mediators that affect glucose uptake and insulin sensitivity in target tissues. Our goal is to identify the metabolic pathways between lipid synthesis and antioxidant defense involved in FAHFA biosynthesis, to examine the substrate specifity of the candidate enzyme, and to introduce a methodology for the quantification of metabolic fluxes through redox reactions linking central carbon metabolism and nicotinamidadene cofactor-driven reductive biosynthesis.
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Achievements
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Our laboratory has been awarded the grant GA20-00317S New metabolic pathway in the synthesis of anti-diabetic lipokines (2020-2022).
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Ondrej Kuda has been awarded Lumina Quaeruntur praemium.
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Publications
Martínez-Ramírez; Felipe - Riečan; Martin - Čajka; Tomáš - Kuda; Ondřej
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Analysis of fatty acid esters of hydroxy fatty acids in edible mushrooms
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LWT-Food Science and Technology. 2023; 173(1 Jan)); 114311
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IF = 6.056
[ASEP]
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doi
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Čajka; Tomáš - Hricko; Jiří - Rudl Kulhavá; Lucie - Paučová; Michaela - Nováková; Michaela - Kuda; Ondřej
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Optimization of Mobile Phase Modifiers for Fast LC-MS-Based Untargeted Metabolomics and Lipidomics
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International Journal of Molecular Sciences. 2023; 24(3)); 1987
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IF = 6.208
[ASEP]
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doi
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Štěpán; M. - Daďová; K. - Matouš; M. - Krauzová; E. - Sontáková; L. - Koc; M. - Larsen; T. - Kuda; Ondřej - Štich; V. - Rossmeislová; L. - Šiklová; M.
Exercise Training Combined with Calanus Oil Supplementation Improves the Central Cardiodynamic Function in Older Women
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Nutrients. 2022; 14(1)); 149
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IF = 6.706
[ASEP]
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doi
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Riečan; Martin - Palůchová; Veronika - Lopes; Magno - Brejchová; Kristýna - Kuda; Ondřej
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Branched and linear fatty acid esters of hydroxy fatty acids (FAHFA) relevant to human health
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Pharmacology and Therapeutics. 2022; 231(Mar)); 107972
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IF = 13.4
[ASEP]
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doi
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Palůchová; Veronika - Čajka; Tomáš - Durand; T. - Vigor; C. - Dodia; Ch. - Chatterjee; S. - Fisher; A. B. - Kuda; Ondřej
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The role of peroxiredoxin 6 in biosynthesis of FAHFAs
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Free Radical Biology and Medicine. Part 2; 193(Nov 20 (2022))
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IF = 8.101
[ASEP]
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doi
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Oeckl; J. - Janovská; Petra - Adamcová; Kateřina - Bardová; Kristina - Brunner; S. - Dieckmann; S. - Ecker; J. - Fromme; T. - Funda; Jiří - Gantert; T. - Giansanti; P. - Soledad Hidrobo; M. - Kuda; Ondřej - Kuster; B. - Li; Y. - Pohl; Radek - Schmitt; S. - Schweizer; S. - Zischka; H. - Zouhar; Petr - Kopecký; Jan - Klingenspor; M.
Loss of UCP1 function augments recruitment of futile lipid cycling for thermogenesis in murine brown fat
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Molecular Metabolism. 2022; 61(July)); 101499
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IF = 8.568
[ASEP]
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doi
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Fisk; H. L. - Childs; C. E. - Miles; E. A. - Ayres; R. - Noakes; P. S. - Paras-Chavez; C. - Kuda; Ondřej - Kopecký; Jan - Antoun; E. - Lillycrop; K. A. - Calder; P. C.
Modification of subcutaneous white adipose tissue inflammation by omega-3 fatty acids is limited in human obesity-a double blind; randomised clinical trial
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EBioMedicine. 2022; 77(Mar)); 103909
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IF = 11.205
[ASEP]
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doi
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Cifarelli; V. - Kuda; Ondřej - Yang; K. - Liu; XP. - Gross; R. W. - Pietka; T. A. - Heo; G. S. - Sultan; D. - Luehmann; H. - Lesser; J. - Ross; M. - Goldberg; I. J. - Gropler; R. J. - Liu; Y. - Abumrad; N. A.
Cardiac immune cell infiltration associates with abnormal lipid metabolism
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Frontiers in Cardiovascular Medicine. 2022; 9(Aug 17)); 948332
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IF = 5.848
[ASEP]
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doi
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Castillo; P. - Kuda; Ondřej - Kopecký; Jan - Pomar; C. A. - Palou; A. - Palou; M. - Picó; C.
Reverting to a Healthy Diet during Lactation Normalizes Maternal Milk Lipid Content of Diet-Induced Obese Rats and Prevents Early Alterations in the Plasma Lipidome of the Offspring
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Molecular Nutrition & Food Research. 2022; 66(17)); 2200204
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IF = 6.575
[ASEP]
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doi
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Brejchová; Kristýna - Palůchová; Veronika - Březinová; Marie - Čajka; Tomáš - Balas; L. - Durand; T. - Křížová; M. - Straňák; Z. - Kuda; Ondřej
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Triacylglycerols containing branched palmitic acid ester of hydroxystearic acid (PAHSA) are present in the breast milk and hydrolyzed by carboxyl ester lipase
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Food Chemistry. 2022; 388(Sep 15)); 132983
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IF = 9.231
[ASEP]
[
doi
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Kristyna Magno Ondrej Veronika Marina
Speedvac
Growing cells
QTRAP 5500 / SelexION
Growing cells
Eolid Phase Extraction
