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Laboratory of Metabolism of Bioactive Lipids

Laboratory of Metabolism of Bioactive Lipids Metabolism of bioactive lipids

The Laboratory focuses on the analysis of lipid mediators (eicosanoids, docosanoids, endocannabinoids) using the UPLC-MS/MS method, in particular to identify the source of production of various mediators and their effect on metabolism and immune cells (i.e. adipocytes vs. macrophages). Currently, the lab is exploring new anti-diabetic lipids, fatty acid esters of hydroxy fatty acids and lipid-related metabolic pathways using stable isotopes and LIMeX platform.

GTTAtlas GTTAtlas - interactive metabolomics and lipidomics atlas of mouse tissues during oral glucose tolerance test (OGTT).

MetaboAtlas21 MetaboAtlas21 - a comprehensive atlas of the mouse metabolome and lipidome.

LORA LORA - a Lipid Over-Representation Analysis tool.

Projects

Branched fatty acid esters of hydroxy fatty acids (FAHFA)

White adipose tissue (WAT) is a complex endocrine organ and its low-grade inflammation in obesity contributes to the development of metabolic disorders. In 2014, a class of WAT-born lipid mediators - fatty acid esters of hydroxy fatty acids (FAHFA) was discovered. FAHFAs are endogenous lipids with anti-inflammatory and anti-diabetic properties, including the enhancement of glucose tolerance, and insulin and glucagon-like peptide 1 (GLP-1) secretion while reducing inflammatory responses [1-5]. More

Omega-3 polyunsaturated fatty acids

Naturally occurring long-chain omega-3 PUFA, namely eicosapentaenoic acid (EPA; 20:5 ω-3), docosapentaenoic acid (DPA; 22:5 ω-3), and docosahexaenoic acid (DHA; 22:6 ω-3) exert multiple biological effects... More

Open science

Our lab participates in OPEN SCIENCE initiative and hosts high school students with their project in metabolomics. More

Chemoinformatics resources

We run local instances of several services focused on Metabolomics, Lipidomics, Statistical analysis and Chemometrics. As the hardware resources are limited, these tools are available only within campus LAN. More

Achievements

Publications

Vondráčková; Michaela - Kopczynski; D. - Hoffmann; N. - Kuda; Ondřej . LORA; Lipid Over-Representation Analysis Based on Structural Information . Analytical Chemistry. 2023; 95(34); 12600-12604 . IF = 7.4 [ASEP] [ doi ]
Song; J. - Duivenvoorde; L. P. M. - Grefte; S. - Kuda; Ondřej - Martínez-Ramírez; Felipe - van der Stelt; I. - Mastorakou; Dimitra - van Schothorst; E. M. - Keijer; J. Normobaric hypoxia shows enhanced FOXO1 signaling in obese mouse gastrocnemius muscle linked to metabolism and muscle structure and neuromuscular innervation . Pflugers Archiv - European Journal of Physiology. 2023; 475(11); 1265-1281 . IF = 4.5 [ASEP] [ doi ]
Martínez-Ramírez; Felipe - Riečan; Martin - Čajka; Tomáš - Kuda; Ondřej . Analysis of fatty acid esters of hydroxy fatty acids in edible mushrooms . LWT-Food Science and Technology. 2023; 173(1 Jan)); 114311 . IF = 6.0 [ASEP] [ doi ]
Hricko; Jiří - Rudl Kulhavá; Lucie - Paučová; Michaela - Nováková; Michaela - Kuda; Ondřej - Fiehn; O. - Čajka; Tomáš . Short-Term Stability of Serum and Liver Extracts for Untargeted Metabolomics and Lipidomics . Antioxidants. 2023; 12(5)); 986 . IF = 7.0 [ASEP] [ doi ]
Drastichová; Z. - Trubačová; Radka - Novotný; J. Regulation of phosphosignaling pathways involved in transcription of cell cycle target genes by TRH receptor activation in GH1 cells . Biomedicine & Pharmacotherapy. 2023; 168(Dec); 115830 . IF = 7.5 [ASEP] [ doi ]
Čajka; Tomáš - Hricko; Jiří - Rudl Kulhavá; Lucie - Paučová; Michaela - Nováková; Michaela - Kuda; Ondřej . Optimization of Mobile Phase Modifiers for Fast LC-MS-Based Untargeted Metabolomics and Lipidomics . International Journal of Molecular Sciences. 2023; 24(3)); 1987 . IF = 5.6 [ASEP] [ doi ]
Čajka; Tomáš - Hricko; Jiří - Rudl Kulhavá; Lucie - Paučová; Michaela - Nováková; Michaela - Fiehn; O. - Kuda; Ondřej . Exploring the Impact of Organic Solvent Quality and Unusual Adduct Formation during LC-MS-Based Lipidomic Profiling . Metabolites. 2023; 13(9); 966 . IF = 4.1 [ASEP] [ doi ]
Castillo; P. - Kuda; Ondřej - Kopecký; Jan - Pomar; C. A. - Palou; A. - Palou; M. - Picó; C. Stachydrine; iN/i-acetylornithine and trimethylamine N-oxide levels as candidate milk biomarkers of maternal consumption of an obesogenic diet during lactation . Biofactors. 2023; 49(5); 1022-1037 . IF = 6.0 [ASEP] [ doi ]
Štěpán; M. - Daďová; K. - Matouš; M. - Krauzová; E. - Sontáková; L. - Koc; M. - Larsen; T. - Kuda; Ondřej - Štich; V. - Rossmeislová; L. - Šiklová; M. Exercise Training Combined with Calanus Oil Supplementation Improves the Central Cardiodynamic Function in Older Women . Nutrients. 2022; 14(1)); 149 . IF = 5.9 [ASEP] [ doi ]
Riečan; Martin - Palůchová; Veronika - Lopes; Magno - Brejchová; Kristýna - Kuda; Ondřej . Branched and linear fatty acid esters of hydroxy fatty acids (FAHFA) relevant to human health . Pharmacology and Therapeutics. 2022; 231(Mar)); 107972 . IF = 13.5 [ASEP] [ doi ]
Palůchová; Veronika - Čajka; Tomáš - Durand; T. - Vigor; C. - Dodia; Ch. - Chatterjee; S. - Fisher; A. B. - Kuda; Ondřej . The role of peroxiredoxin 6 in biosynthesis of FAHFAs . Free Radical Biology and Medicine. Part 2; 193(Nov 20 (2022)) . IF = 7.4 [ASEP] [ doi ]
Oeckl; J. - Janovská; Petra - Adamcová; Kateřina - Bardová; Kristina - Brunner; S. - Dieckmann; S. - Ecker; J. - Fromme; T. - Funda; Jiří - Gantert; T. - Giansanti; P. - Soledad Hidrobo; M. - Kuda; Ondřej - Kuster; B. - Li; Y. - Pohl; Radek - Schmitt; S. - Schweizer; S. - Zischka; H. - Zouhar; Petr - Kopecký; Jan - Klingenspor; M. Loss of UCP1 function augments recruitment of futile lipid cycling for thermogenesis in murine brown fat . Molecular Metabolism. 2022; 61(July)); 101499 . IF = 8.1 [ASEP] [ doi ]
Fisk; H. L. - Childs; C. E. - Miles; E. A. - Ayres; R. - Noakes; P. S. - Paras-Chavez; C. - Kuda; Ondřej - Kopecký; Jan - Antoun; E. - Lillycrop; K. A. - Calder; P. C. Modification of subcutaneous white adipose tissue inflammation by omega-3 fatty acids is limited in human obesity-a double blind; randomised clinical trial . EBioMedicine. 2022; 77(Mar)); 103909 . IF = 11.1 [ASEP] [ doi ]
Cifarelli; V. - Kuda; Ondřej - Yang; K. - Liu; XP. - Gross; R. W. - Pietka; T. A. - Heo; G. S. - Sultan; D. - Luehmann; H. - Lesser; J. - Ross; M. - Goldberg; I. J. - Gropler; R. J. - Liu; Y. - Abumrad; N. A. Cardiac immune cell infiltration associates with abnormal lipid metabolism . Frontiers in Cardiovascular Medicine. 2022; 9(Aug 17)); 948332 . IF = 3.6 [ASEP] [ doi ]
Castillo; P. - Kuda; Ondřej - Kopecký; Jan - Pomar; C. A. - Palou; A. - Palou; M. - Picó; C. Reverting to a Healthy Diet during Lactation Normalizes Maternal Milk Lipid Content of Diet-Induced Obese Rats and Prevents Early Alterations in the Plasma Lipidome of the Offspring . Molecular Nutrition & Food Research. 2022; 66(17)); 2200204 . IF = 5.2 [ASEP] [ doi ]

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People

Ondrej Kuda, Ph.D.
Head of the Laboratory
Kristyna Brejchova, Ph.D.
Scientist
Dovilė Milonaitytė, PhD
postdoc
Mgr. Martin Riečan
Postgradual Student
Jana Brejchová, Ph.D.
labmanager
Mgr. Martina Rombaldová, Ph.D.
labmanager
Michaela Vondráčková, MSc.
bioinformatics
Monika Krakovkova
student