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Laboratory of Metabolism of Bioactive Lipids

Laboratory of Metabolism of Bioactive Lipids Metabolism of bioactive lipids

The Laboratory focuses on the analysis of lipid mediators (eicosanoids, docosanoids, endocannabinoids) using the UPLC-MS/MS method, in particular to identify the source of production of various mediators and their effect on metabolism and immune cells (i.e. adipocytes vs. macrophages). Currently, the lab is exploring new anti-diabetic lipids, fatty acid esters of hydroxy fatty acids and lipid-related metabolic pathways using stable isotopes and LIMeX platform.

GTTAtlas GTTAtlas - interactive metabolomics and lipidomics atlas of mouse tissues during oral glucose tolerance test (OGTT).

MetaboAtlas21 MetaboAtlas21 - a comprehensive atlas of the mouse metabolome and lipidome.

Projects

Branched fatty acid esters of hydroxy fatty acids (FAHFA)

White adipose tissue (WAT) is a complex endocrine organ and its low-grade inflammation in obesity contributes to the development of metabolic disorders. In 2014, a class of WAT-born lipid mediators - fatty acid esters of hydroxy fatty acids (FAHFA) was discovered. FAHFAs are endogenous lipids with anti-inflammatory and anti-diabetic properties, including the enhancement of glucose tolerance, and insulin and glucagon-like peptide 1 (GLP-1) secretion while reducing inflammatory responses [1-5]. More

Omega-3 polyunsaturated fatty acids

Naturally occurring long-chain omega-3 PUFA, namely eicosapentaenoic acid (EPA; 20:5 ω-3), docosapentaenoic acid (DPA; 22:5 ω-3), and docosahexaenoic acid (DHA; 22:6 ω-3) exert multiple biological effects... More

Open science

Our lab participates in OPEN SCIENCE initiative and hosts high school students with their project in metabolomics. More

Chemoinformatics resources

We run local instances of several services focused on Metabolomics, Lipidomics, Statistical analysis and Chemometrics. As the hardware resources are limited, these tools are available only within campus LAN. More

Achievements

Publications

Štěpán; M. - Daďová; K. - Matouš; M. - Krauzová; E. - Sontáková; L. - Koc; M. - Larsen; T. - Kuda; Ondřej - Štich; V. - Rossmeislová; L. - Šiklová; M. Exercise Training Combined with Calanus Oil Supplementation Improves the Central Cardiodynamic Function in Older Women . Nutrients. 2022; 14(1)); 149 . IF = 6.706 [ASEP] [ doi ]
Riečan; Martin - Palůchová; Veronika - Lopes; Magno - Brejchová; Kristýna - Kuda; Ondřej . Branched and linear fatty acid esters of hydroxy fatty acids (FAHFA) relevant to human health . Pharmacology and Therapeutics. 2022; 231(Mar)); 107972 . IF = 13.4 [ASEP] [ doi ]
Oeckl; J. - Janovská; Petra - Adamcová; Kateřina - Bardová; Kristina - Brunner; S. - Dieckmann; S. - Ecker; J. - Fromme; T. - Funda; Jiří - Gantert; T. - Giansanti; P. - Soledad Hidrobo; M. - Kuda; Ondřej - Kuster; B. - Li; Y. - Pohl; Radek - Schmitt; S. - Schweizer; S. - Zischka; H. - Zouhar; Petr - Kopecký; Jan - Klingenspor; M. Loss of UCP1 function augments recruitment of futile lipid cycling for thermogenesis in murine brown fat . Molecular Metabolism. 2022; 61(July)); 101499 . IF = 8.568 [ASEP] [ doi ]
Fisk; H. L. - Childs; C. E. - Miles; E. A. - Ayres; R. - Noakes; P. S. - Paras-Chavez; C. - Kuda; Ondřej - Kopecký; Jan - Antoun; E. - Lillycrop; K. A. - Calder; P. C. Modification of subcutaneous white adipose tissue inflammation by omega-3 fatty acids is limited in human obesity-a double blind; randomised clinical trial . EBioMedicine. 2022; 77(Mar)); 103909 . IF = 11.205 [ASEP] [ doi ]
Brejchová; Kristýna - Palůchová; Veronika - Březinová; Marie - Čajka; Tomáš - Balas; L. - Durand; T. - Křížová; M. - Straňák; Z. - Kuda; Ondřej . Triacylglycerols containing branched palmitic acid ester of hydroxystearic acid (PAHSA) are present in the breast milk and hydrolyzed by carboxyl ester lipase . Food Chemistry. 2022; 388(Sep 15)); 132983 . IF = 9.231 [ASEP] [ doi ]
Sistilli; Gabriella - Kalendová; Veronika - Čajka; Tomáš - Irodenko; Ilaria - Bardová; Kristina - Oseeva; Marina - Žáček; P. - Kroupová; Petra - Horáková; Olga - Lackner; K. - Gastaldelli; A. - Kuda; Ondřej - Kopecký; Jan - Rossmeisl; Martin . Krill Oil Supplementation Reduces Exacerbated Hepatic Steatosis Induced by Thermoneutral Housing in Mice with Diet-Induced Obesity . Nutrients. 2021; 13(2)); 437 . IF = 6.706 [ASEP] [ doi ]
Lopes; Magno Alves - Brejchová; Kristýna - Riečan; Martin - Nováková; Michaela - Rossmeisl; Martin - Čajka; Tomáš - Kuda; Ondřej . Metabolomics atlas of oral 13C-glucose tolerance test in mice . Cell Reports. 2021; 37(2)); 109833 . IF = 9.995 [ASEP] [ doi ]
Leal; L. G. - Lopes; Magno Alves - Peres; S. B. - Batista; M. L.; Jr. Exercise Training as Therapeutic Approach in Cancer Cachexia: A Review of Potential Anti-inflammatory Effect on Muscle Wasting . Frontiers in physiology. 2021; 11(Feb 4)); 570170 . IF = 4.755 [ASEP] [ doi ]
Jacome-Sosa; M. - Miao; Z.-F. - Peche; V. S. - Morris; E. F. - Narendran; R. - Pietka; K. M. - Samovski; D. - Lo; H.-Y. G. - Pietka; T. - Varro; A. - Love-Gregory; L. - Goldenring; J. R. - Kuda; Ondřej - Gamanzon; E. R. - Mills; J. C. - Abumrad; N. A. CD36 maintains the gastric mucosa and associates with gastric disease . Communications Biology. 2021; 4(1)); 1247 . IF = 6.548 [ASEP] [ doi ]
Fisk; H. L. - Childs; C. E. - Miles; E. A. - Ayres; R. - Noakes; P. S. - Paras-Chaves; C. - Kuda; Ondřej - Kopecký; Jan - Antoun; E. - Lillycrop; K. A. - Calder; P. C. Dysregulation of endocannabinoid concentrations in human subcutaneous adipose tissue in obesity and modulation by omega-3 polyunsaturated fatty acids . Clinical science. 2021; 135(1); 185-200 . IF = 6.876 [ASEP] [ doi ]
Daňhelovská; T. - Zdražilová; L. - Štufková; H. - Vanišová; M. - Volfová; N. - Křížová; J. - Kuda; Ondřej - Sládková; J. - Tesařová; M. Knock-Out of ACBD3 Leads to Dispersed Golgi Structure; but Unaffected Mitochondrial Functions in HEK293 and HeLa Cells . International Journal of Molecular Sciences. 2021; 22(14)); 7270 . IF = 6.208 [ASEP] [ doi ]
Bugajev; Viktor - Paulenda; Tomáš - Utekal; Pavol - Mrkáček; Michal - Hálová; Ivana - Kuchař; L. - Kuda; Ondřej - Vávrová; Petra - Schuster; Bjorn - Fuentes-Liso; Sergio - Potůčková; Lucie - Smrž; D. - Černohouzová; Sára - Dráberová; Lubica - Bambousková; Monika - Dráber; Petr . Crosstalk between ORMDL3; serine palmitoyltransferase; and 5-lipoxygenase in the sphingolipid and eicosanoid metabolic pathways . Journal of Lipid Research. 2021; 62(September)); 100121 . IF = 6.676 [ASEP] [ doi ]
Brejchová; Kristýna - Radner; F. P. W. - Balas; L. - Palůchová; Veronika - Čajka; Tomáš - Chodounská; Hana - Kudová; Eva - Schratter; M. - Schreiber; R. - Durand; T. - Zechner; R. - Kuda; Ondřej . Distinct roles of adipose triglyceride lipase and hormone-sensitive lipase in the catabolism of triacylglycerol estolides . Proceedings of the National Academy of Sciences of the United States of America. 2021; 118(2)); e2020999118 . IF = 12.779 [ASEP] [ doi ]
Benlebna; M. - Balas; L. - Bonafos; B. - Pessemesse; L. - Fouret; G. - Vigor; C. - Gaillet; S. - Grober; J. - Bernex; F. - Landrier; J.F. - Kuda; Ondřej - Durand; T. - Coudray; Ch. - Casas; F. - Feillet-Coudray; Ch. Long-term intake of 9-PAHPA or 9-OAHPA modulates favorably the basal metabolism and exerts an insulin sensitizing effect in obesogenic diet-fed mice . European Journal of Nutrition. 2021; 60(4); 2013-2027 . IF = 4.868 [ASEP] [ doi ]
van der Hoek; M. D. - Nieuwenhuizen; A. G. - Kuda; Ondřej - Bos; P. - Palůchová; Veronika - Verschuren; L. - van den Hoek; A. M. - Kleemann; R. - Veeger; N. J. G. M. - van der Leij; F. R. - Keijer; J. Intramuscular short-chain acylcarnitines in elderly people are decreased in (pre-)frail females; but not in males . FASEB Journal. 2020; 34(9); 11658-11671 . IF = 5.191 [ASEP] [ doi ]
Rossmeisl; Martin - Pavlišová; Jana - Bardová; Kristina - Kalendová; Veronika - Burešová; Jana - Kuda; Ondřej - Kroupová; Petra - Staňková; B. - Tvrzická; E. - Fišerová; E. - Horáková; Olga - Kopecký; Jan . Increased plasma levels of palmitoleic acid may contribute to beneficial effects of Krill oil on glucose homeostasis in dietary obese mice . Biochimica Et Biophysica Acta-Molecular and Cell Biology of Lipids. 2020; 1865(8)); 158732 . IF = 4.698 [ASEP] [ doi ]
Pomar; C. A. - Kuda; Ondřej - Kopecký; Jan - Rombaldová; Martina - Castro; H. - Picó; C. - Sánchez; J. - Palou; A. Maternal diet; rather than obesity itself; has a main influence on milk triacylglycerol profile in dietary obese rats . Biochimica Et Biophysica Acta-Molecular and Cell Biology of Lipids. 2020; 1865(2)); 158556 . IF = 4.698 [ASEP] [ doi ]
Palůchová; Veronika - Vik; A. - Čajka; Tomáš - Březinová; Marie - Brejchová; Kristýna - Bugajev; Viktor - Dráberová; Lubica - Dráber; Peter - Burešová; Jana - Kroupová; Petra - Bardová; Kristina - Rossmeisl; Martin - Kopecký; Jan - Hansen; T. V. - Kuda; Ondřej . Triacylglycerol-Rich Oils of Marine Origin are Optimal Nutrients for Induction of Polyunsaturated Docosahexaenoic Acid Ester of Hydroxy Linoleic Acid (13-DHAHLA) with Anti-Inflammatory Properties in Mice . Molecular Nutrition & Food Research. 2020; 64(11)); 1901238 . IF = 5.914 [ASEP] [ doi ]
Palůchová; Veronika - Oseeva; Marina - Březinová; Marie - Čajka; Tomáš - Bardová; Kristina - Adamcová; Kateřina - Žáček; Petr - Brejchová; Kristýna - Balas; L. - Chodounská; Hana - Kudová; Eva - Schreiber; R. - Zechner; R. - Durand; T. - Rossmeisl; Martin - Abumrad; N. A. - Kopecký; Jan - Kuda; Ondřej . Lipokine 5-PAHSA is Regulated by Adipose Triglyceride Lipase and Primes Adipocytes for de novo Lipogenesis in Mice . Diabetes. 2020; 69(3); 300-312 . IF = 9.461 [ASEP] [ doi ]
Kroupová; Petra - van Schothorst; E. M. - Keijer; J. - Bunschoten; A. - Vodička; Martin - Irodenko; Ilaria - Oseeva; Marina - Žáček; P. - Kopecký; Jan - Rossmeisl; Martin - Horáková; Olga . Omega-3 Phospholipids from Krill Oil Enhance Intestinal Fatty Acid Oxidation More Effectively than Omega-3 Triacylglycerols in High-Fat Diet-Fed Obese Mice . Nutrients. 2020; 12(7)); 2037 . IF = 5.717 [ASEP] [ doi ]

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People

Ondrej Kuda, Ph.D.
Head of the Laboratory
Kristyna Brejchova, Ph.D.
Scientist
Ing. Veronika Paluchova
Postgradual Student
Mgr. Martin Riečan
Postgradual Student
Felipe Martínez Ramírez, MSc.
postgradual student
Radka Trubačová, MSc.
labmanager