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Laboratory of Pain Research

Laboratory of Pain Research Neuronal activity recorded in spinal cord (A) by patch-clamp (B). Optical stimulation of neurons with chanelorhodopsin (C) evokes inhibitory currents in excitatory neurons (D) that are reduced under pathological conditions (E) and contribute to pain.

The main research interest of our Laboratory is to study mechanisms of pain and to explore new possibilities of pain treatment, especially in chronic states. Our experimental work is concentrated on the modulation of nociceptive information at the spinal cord level that is the relay center between the periphery and the higher brain areas. Our goal is to study these modulatory mechanisms in order to improve therapy for pain conditions, such as allodynia, hyperalgesia, neuropathic and cancer related pain. Our focus is on the role of TRPV1, purinergic P2X receptors and inflammatory cytokines. In our research we use mainly electrophysiological, optogenetic, immunohistochemical and behavioral methods.

The cellular and molecular neuroendocrinology group, led by Dr. Zemkova, is studying the relationship between the structure and function of P2X receptor channels and their role in electrical activity, calcium signaling, neurotransmitter and hormone release in neuroendocrine cells.

Projects

Publications

Cimická; Jana - Riegert; J. - Kavková; M. - Černá; K. Intestinal mycobiome associated with diagnosis of inflammatory bowel disease based on tissue biopsies . Medical Mycology. 2022; 60(1)); myab076 . IF = 3.747 [ASEP] [ doi ]
Adámek; Pavel - Heleš; Mário - Bhattacharyya; Anirban - Pontearso; Monica - Slepicka; Jakub - Paleček; Jiří . Dual PI3K?/? Inhibitor Duvelisib Prevents Development of Neuropathic Pain in Model of Paclitaxel-Induced Peripheral Neuropathy . Journal of Neuroscience. 2022; 42(9); 1864-1881 . IF = 6.709 [ASEP] [ doi ]
Zemková; Hana . Special Issue of International Journal of Molecular Sciences (IJMS) "Purinergic P2 Receptors: Structure and Function" . International Journal of Molecular Sciences. 2021; 22(1)); 383 . IF = 6.208 [ASEP] [ doi ]
Uchytilová; Eva - Špicarová; Diana - Paleček; Jiří . Hypersensitivity Induced by Intrathecal Bradykinin Administration Is Enhanced by N-oleoyldopamine (OLDA) and Prevented by TRPV1 Antagonist . International Journal of Molecular Sciences. 2021; 22(7)); 3712 . IF = 6.208 [ASEP] [ doi ]
Mrózková; Petra - Špicarová; Diana - Paleček; Jiří . Spinal PAR2 Activation Contributes to Hypersensitivity Induced by Peripheral Inflammation in Rats . International Journal of Molecular Sciences. 2021; 22(3)); 991 . IF = 6.208 [ASEP] [ doi ]
Moravcová; S. - Filipovská; E. - Spišská; V. - Svobodová; Irena - Novotný; J. - Bendová; Z. The Circadian Rhythms of STAT3 in the Rat Pineal Gland and Its Involvement in Arylalkylamine-N-Acetyltransferase Regulation . Life. 2021; 11(10)); 1105 . IF = 3.251 [ASEP] [ doi ]
Míková; H. - Kuchtiak; Viktor - Svobodová; Irena - Spišská; V. - Pačesová; D. - Balík; Aleš - Bendová; Z. Circadian Regulation of GluA2 mRNA Processing in the Rat Suprachiasmatic Nucleus and Other Brain Structures . Molecular Neurobiology. 2021; 58(1); 439-449 . IF = 5.682 [ASEP] [ doi ]
Heleš; Mário - Mrózková; Petra - Šulcová; Dominika - Adámek; Pavel - Špicarová; Diana - Paleček; Jiří . Chemokine CCL2 prevents opioid-induced inhibition of nociceptive synaptic transmission in spinal cord dorsal horn . Journal of Neuroinflammation. 2021; 18(1)); 279 . IF = 9.587 [ASEP] [ doi ]
Sivčev; Sonja - Slavíková; Barbora - Ivetic; Milorad - Knězů; Michal - Kudová; Eva - Zemková; Hana . Lithocholic acid inhibits P2X2 and potentiates P2X4 receptor channel gating . Journal of Steroid Biochemistry and Molecular Biology. 2020; 202(Sep)); 105725 . IF = 4.292 [ASEP] [ doi ]
Rupert; Marian - Bhattacharya; Anirban - Stillerová Tvrdoňová; Vendula - Jindřichová; Marie - Mokdad; Audrey - Boué-Grabot; E. - Zemková; Hana . Role of Conserved Residues and F322 in the Extracellular Vestibule of the Rat P2X7 Receptor in Its Expression; Function and Dye Uptake Ability . International Journal of Molecular Sciences. 2020; 21(22)); 8446 . IF = 5.923 [ASEP] [ doi ]
Kalynovska; Nataliia - Diallo; Mickael - Sotáková-Kašparová; Dita - Paleček; Jiří . Losartan attenuates neuroinflammation and neuropathic pain in paclitaxel-induced peripheral neuropathy . Journal of Cellular and Molecular Medicine. 2020; 24(14); 7949-7958 . IF = 5.310 [ASEP] [ doi ]
Sýkora; M. - Szeiffová Bačová; B. - Egan Beňová; T. - Barančík; M. - Žurmanová; J. - Rauchová; Hana - Weismann; P. - Pavelka; Stanislav - Kurahara; L. H. - Slezák; J. - Soukup; Tomáš - Tribulová; N. Cardiac Cx43 and ECM Responses to Altered Thyroid Status Are Blunted in Spontaneously Hypertensive versus Normotensive Rats . International Journal of Molecular Sciences. 2019; 20(15)); 3758 . IF = 4.556 [ASEP] [ doi ]
Sivčev; Sonja - Slavíková; Barbora - Rupert; Marian - Ivetic; Milorad - Nekardová; Michaela - Kudová; Eva - Zemková; Hana . Synthetic testosterone derivatives modulate rat P2X2 and P2X4 receptor channel gating . Journal of Neurochemistry. 2019; 150(1); 28-43 . IF = 4.066 [ASEP] [ doi ]
Kalynovska, Nataliia - Diallo, Michael - Paleček, Jiří . Losartan treatment attenuates the development of neuropathic thermal hyperalgesia induced by peripheral nerve injury in rats . Life Sciences 2019, 220(Mar 1), 147-155 . IF = 3.647 [ASEP] [ doi ]
Ivetic; Milorad - Bhattacharya; Anirban - Zemková; Hana . P2X2 Receptor Expression and Function Is Upregulated in the Rat Supraoptic Nucleus Stimulated Through Refeeding After Fasting . Frontiers in Cellular Neuroscience. 2019; 13(Jun 26)); 284 . IF = 3.921 [ASEP] [ doi ]
Adámek, Pavel - Heleš, Mário - Paleček, Jiří . Mechanical allodynia and enhanced responses to capsaicin are mediated by PI3K in a paclitaxel model of peripheral neuropathy . Neuropharmacology 2019, 146(Mar 1), 163-174 . IF = 4.431 [ASEP] [ doi ]
Zemková, Hana - Stojilkovic, S. S. Neurotransmitter receptors as signaling platforms in anterior pituitary cells . Molecular and Cellular Endocrinology 2018, roč. 463, C, p. 49-64 . IF = 3.693 [ASEP] [ doi ]
Torres-Pérez, J. V. - Adámek, Pavel - Paleček, Jiří - Vizcaychipi, M. - Nagy, I. - Varga, A. The NA(v)1.7 blocker protoxin II reduces burn injury-induced spinal nociceptive processing . Journal of Molecular Medicine-Jmm 2018, roč. 96, 1, p. 75-84 . IF = 4.746 [ASEP] [ doi ]
Svobodová, Irena - Bhattacharya, Anirban - Ivetic, Milorad - Bendová, Z. - Zemková, Hana . Circadian ATP Release in Organotypic Cultures of the Rat Suprachiasmatic Nucleus Is Dependent on P2X7 and P2Y Receptors . Frontiers in Pharmacology 2018, roč. 9, Mar 6, článku 192 . IF = 3.845 [ASEP] [ doi ]
Rauchová, Hana - Vokurková, Martina - Pavelka, Stanislav - Vaněčková, Ivana - Tribulová, N. - Soukup, Tomáš . Red palm oil supplementation does not increase blood glucose or serum lipids levels in Wistar rats with different thyroid status . Physiological Research 2018, roč. 67, 2, p. 307-315 . IF = 1.701 [ASEP]
Nerandžič, Vladimír - Mrózková, Petra - Adámek, Pavel - Špicarová, Diana - Nagy, I. - Paleček, Jiří . Peripheral inflammation affects modulation of nociceptive synaptic transmission in the spinal cord induced by N‐arachidonoylphosphatidylethanolamine . British Journal of Pharmacology 2018, roč. 175, 12, p. 2322-2356 . IF = 6.583 [ASEP] [ doi ]
Szeiffová Bačová, B. - Vinczenzová, C. - Žurmanová, J. - Kašparová, Dita - Knezl, V. - Egan Beňová, T. - Pavelka, Stanislav - Soukup, Tomáš - Tribulová, N. Altered thyroid status affects myocardial expression of connexin-43 and susceptibility of rat heart to malignant arrhythmias that can be partially normalized by red palm oil intake . Histochemistry and Cell Biology. 2017, roč. 147, 1, p. 63-73 . IF = 2.164 [ASEP] [ doi ]
Stojilkovic, S. S. - Bjelobaba, I. - Zemková, Hana . Ion Channels of Pituitary Gonadotrophs and Their Roles in Signaling and Secretion . Frontiers in Endocrinology. 2017, roč. 8, Jun 9, článku 126 . IF = 3.519 [ASEP] [ doi ]
Mackay, L. - Zemková, Hana - Stojilkovic, S. S. - Sherman, A. - Khadra, A. Deciphering the regulation of P2X4 receptor channel gating by ivermectin using Markov models . PLoS Computational Biology. 2017, roč. 13, 7, článku e1005643 . IF = 3.955 [ASEP] [ doi ]
Kalynovska, Nataliia - Adámek, Pavel - Paleček, Jiří . TRPV1 receptors contribute to mediate paclitaxel-induced c-Fos expression in spinal cord dorsal horn neurons . Physiological Research. 2017, roč. 66, 3, p. 549-532 . IF = 1.324 [ASEP]

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People

Jiří Paleček, MD, PhD
Head of the Laboratory
Mgr. Diana Špicarová, Ph.D.
Deputy Head of the Laboratory
RNDr. Hana Zemková, CSc.
Senior Researcher
Anirban Bhattacharya, Ph.D.
Senior Researcher
Mgr. Petra Mrózková, Ph.D.
Postdoctoral Fellow
RNDr. Pavel Adámek, Ph.D.
Postdoctoral Fellow
Mgr. Mario Heleš
PhD Student
Mgr. Jakub Slepička
PhD Student
Mgr. Monica Pontearso
PhD Student
  Mgr. Marian Rupert
PhD Student
  Mgr. Sonja Sivcev
PhD Student
 

Mgr. Jana Cimická

PhD Student

 

Mgr. Daniel Vasconcelos

PhD Student

 

David Mužík

Student

 

Anna Kratinová

Student

 

Denisa Dresslerová

Student

Ing. Hana Janoušková
Technician
Kateřina Kramerová
Technician