Metabolism of bioactive lipids
The Laboratory focuses on the analysis of lipid mediators (eicosanoids, docosanoids, endocannabinoids) using the UPLC-MS/MS method, in particular to identify the source of production of various mediators and their effect on metabolism and immune cells (i.e. adipocytes vs. macrophages). Currently, the lab is exploring new anti-diabetic lipids, fatty acid esters of hydroxy fatty acids and lipid-related metabolic pathways using stable isotopes and LIMeX platform.
GTTAtlas - interactive metabolomics and lipidomics atlas of mouse tissues during oral glucose tolerance test (OGTT).
MetaboAtlas21 - a comprehensive atlas of the mouse metabolome and lipidome.
LORA - a Lipid Over-Representation Analysis tool.
Projects
Software:
GTTAtlas
MetaboAtlas21
Mapper
Reaction network viewer
LORA
Methodological highlighs from publications using small molecule omics techniques.
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White adipose tissue (WAT) is a complex endocrine organ and its low-grade inflammation in obesity contributes to the development of metabolic disorders. In 2014, a class of WAT-born lipid mediators - fatty acid esters of hydroxy fatty acids (FAHFA) was discovered. FAHFAs are endogenous lipids with anti-inflammatory and anti-diabetic properties, including the enhancement of glucose tolerance, and insulin and glucagon-like peptide 1 (GLP-1) secretion while reducing inflammatory responses [1-5].
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Naturally occurring long-chain omega-3 PUFA, namely eicosapentaenoic acid (EPA; 20:5 ω-3), docosapentaenoic acid (DPA; 22:5 ω-3), and docosahexaenoic acid (DHA; 22:6 ω-3) exert multiple biological effects...
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Our lab participates in OPEN SCIENCE initiative and hosts high school students with their project in metabolomics.
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We run local instances of several services focused on Metabolomics, Lipidomics, Statistical analysis and Chemometrics. As the hardware resources are limited, these tools are available only within campus LAN.
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Achievements
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Our laboratory has been awarded the grant GA20-00317S New metabolic pathway in the synthesis of anti-diabetic lipokines (2020-2022).
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Ondrej Kuda has been awarded Lumina Quaeruntur praemium.
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Publications
Martínez-Ramírez; Felipe - Riečan; Martin - Čajka; Tomáš - Kuda; Ondřej
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Analysis of fatty acid esters of hydroxy fatty acids in edible mushrooms
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LWT-Food Science and Technology. 2023; 173(1 Jan)); 114311
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IF = 6.056
[ASEP]
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doi
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Čajka; Tomáš - Hricko; Jiří - Rudl Kulhavá; Lucie - Paučová; Michaela - Nováková; Michaela - Kuda; Ondřej
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Optimization of Mobile Phase Modifiers for Fast LC-MS-Based Untargeted Metabolomics and Lipidomics
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International Journal of Molecular Sciences. 2023; 24(3)); 1987
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IF = 6.208
[ASEP]
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doi
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Štěpán; M. - Daďová; K. - Matouš; M. - Krauzová; E. - Sontáková; L. - Koc; M. - Larsen; T. - Kuda; Ondřej - Štich; V. - Rossmeislová; L. - Šiklová; M.
Exercise Training Combined with Calanus Oil Supplementation Improves the Central Cardiodynamic Function in Older Women
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Nutrients. 2022; 14(1)); 149
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IF = 6.706
[ASEP]
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doi
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Riečan; Martin - Palůchová; Veronika - Lopes; Magno - Brejchová; Kristýna - Kuda; Ondřej
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Branched and linear fatty acid esters of hydroxy fatty acids (FAHFA) relevant to human health
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Pharmacology and Therapeutics. 2022; 231(Mar)); 107972
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IF = 13.4
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doi
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Palůchová; Veronika - Čajka; Tomáš - Durand; T. - Vigor; C. - Dodia; Ch. - Chatterjee; S. - Fisher; A. B. - Kuda; Ondřej
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The role of peroxiredoxin 6 in biosynthesis of FAHFAs
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Free Radical Biology and Medicine. Part 2; 193(Nov 20 (2022))
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IF = 8.101
[ASEP]
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doi
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Oeckl; J. - Janovská; Petra - Adamcová; Kateřina - Bardová; Kristina - Brunner; S. - Dieckmann; S. - Ecker; J. - Fromme; T. - Funda; Jiří - Gantert; T. - Giansanti; P. - Soledad Hidrobo; M. - Kuda; Ondřej - Kuster; B. - Li; Y. - Pohl; Radek - Schmitt; S. - Schweizer; S. - Zischka; H. - Zouhar; Petr - Kopecký; Jan - Klingenspor; M.
Loss of UCP1 function augments recruitment of futile lipid cycling for thermogenesis in murine brown fat
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Molecular Metabolism. 2022; 61(July)); 101499
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IF = 8.568
[ASEP]
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doi
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Fisk; H. L. - Childs; C. E. - Miles; E. A. - Ayres; R. - Noakes; P. S. - Paras-Chavez; C. - Kuda; Ondřej - Kopecký; Jan - Antoun; E. - Lillycrop; K. A. - Calder; P. C.
Modification of subcutaneous white adipose tissue inflammation by omega-3 fatty acids is limited in human obesity-a double blind; randomised clinical trial
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EBioMedicine. 2022; 77(Mar)); 103909
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IF = 11.205
[ASEP]
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doi
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Cifarelli; V. - Kuda; Ondřej - Yang; K. - Liu; XP. - Gross; R. W. - Pietka; T. A. - Heo; G. S. - Sultan; D. - Luehmann; H. - Lesser; J. - Ross; M. - Goldberg; I. J. - Gropler; R. J. - Liu; Y. - Abumrad; N. A.
Cardiac immune cell infiltration associates with abnormal lipid metabolism
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Frontiers in Cardiovascular Medicine. 2022; 9(Aug 17)); 948332
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IF = 5.848
[ASEP]
[
doi
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Castillo; P. - Kuda; Ondřej - Kopecký; Jan - Pomar; C. A. - Palou; A. - Palou; M. - Picó; C.
Reverting to a Healthy Diet during Lactation Normalizes Maternal Milk Lipid Content of Diet-Induced Obese Rats and Prevents Early Alterations in the Plasma Lipidome of the Offspring
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Molecular Nutrition & Food Research. 2022; 66(17)); 2200204
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IF = 6.575
[ASEP]
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doi
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Brejchová; Kristýna - Palůchová; Veronika - Březinová; Marie - Čajka; Tomáš - Balas; L. - Durand; T. - Křížová; M. - Straňák; Z. - Kuda; Ondřej
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Triacylglycerols containing branched palmitic acid ester of hydroxystearic acid (PAHSA) are present in the breast milk and hydrolyzed by carboxyl ester lipase
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Food Chemistry. 2022; 388(Sep 15)); 132983
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IF = 9.231
[ASEP]
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doi
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Sistilli; Gabriella - Kalendová; Veronika - Čajka; Tomáš - Irodenko; Ilaria - Bardová; Kristina - Oseeva; Marina - Žáček; P. - Kroupová; Petra - Horáková; Olga - Lackner; K. - Gastaldelli; A. - Kuda; Ondřej - Kopecký; Jan - Rossmeisl; Martin
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Krill Oil Supplementation Reduces Exacerbated Hepatic Steatosis Induced by Thermoneutral Housing in Mice with Diet-Induced Obesity
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Nutrients. 2021; 13(2)); 437
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IF = 6.706
[ASEP]
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doi
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Lopes; Magno Alves - Brejchová; Kristýna - Riečan; Martin - Nováková; Michaela - Rossmeisl; Martin - Čajka; Tomáš - Kuda; Ondřej
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Metabolomics atlas of oral 13C-glucose tolerance test in mice
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Cell Reports. 2021; 37(2)); 109833
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IF = 9.995
[ASEP]
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doi
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Leal; L. G. - Lopes; Magno Alves - Peres; S. B. - Batista; M. L.; Jr.
Exercise Training as Therapeutic Approach in Cancer Cachexia: A Review of Potential Anti-inflammatory Effect on Muscle Wasting
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Frontiers in physiology. 2021; 11(Feb 4)); 570170
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IF = 4.755
[ASEP]
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doi
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Jacome-Sosa; M. - Miao; Z.-F. - Peche; V. S. - Morris; E. F. - Narendran; R. - Pietka; K. M. - Samovski; D. - Lo; H.-Y. G. - Pietka; T. - Varro; A. - Love-Gregory; L. - Goldenring; J. R. - Kuda; Ondřej - Gamanzon; E. R. - Mills; J. C. - Abumrad; N. A.
CD36 maintains the gastric mucosa and associates with gastric disease
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Communications Biology. 2021; 4(1)); 1247
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IF = 6.548
[ASEP]
[
doi
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Fisk; H. L. - Childs; C. E. - Miles; E. A. - Ayres; R. - Noakes; P. S. - Paras-Chaves; C. - Kuda; Ondřej - Kopecký; Jan - Antoun; E. - Lillycrop; K. A. - Calder; P. C.
Dysregulation of endocannabinoid concentrations in human subcutaneous adipose tissue in obesity and modulation by omega-3 polyunsaturated fatty acids
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Clinical science. 2021; 135(1); 185-200
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IF = 6.876
[ASEP]
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doi
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Daňhelovská; T. - Zdražilová; L. - Štufková; H. - Vanišová; M. - Volfová; N. - Křížová; J. - Kuda; Ondřej - Sládková; J. - Tesařová; M.
Knock-Out of ACBD3 Leads to Dispersed Golgi Structure; but Unaffected Mitochondrial Functions in HEK293 and HeLa Cells
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International Journal of Molecular Sciences. 2021; 22(14)); 7270
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IF = 6.208
[ASEP]
[
doi
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Bugajev; Viktor - Paulenda; Tomáš - Utekal; Pavol - Mrkáček; Michal - Hálová; Ivana - Kuchař; L. - Kuda; Ondřej - Vávrová; Petra - Schuster; Bjorn - Fuentes-Liso; Sergio - Potůčková; Lucie - Smrž; D. - Černohouzová; Sára - Dráberová; Lubica - Bambousková; Monika - Dráber; Petr
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Crosstalk between ORMDL3; serine palmitoyltransferase; and 5-lipoxygenase in the sphingolipid and eicosanoid metabolic pathways
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Journal of Lipid Research. 2021; 62(September)); 100121
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IF = 6.676
[ASEP]
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doi
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Brejchová; Kristýna - Radner; F. P. W. - Balas; L. - Palůchová; Veronika - Čajka; Tomáš - Chodounská; Hana - Kudová; Eva - Schratter; M. - Schreiber; R. - Durand; T. - Zechner; R. - Kuda; Ondřej
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Distinct roles of adipose triglyceride lipase and hormone-sensitive lipase in the catabolism of triacylglycerol estolides
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Proceedings of the National Academy of Sciences of the United States of America. 2021; 118(2)); e2020999118
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IF = 12.779
[ASEP]
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doi
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Benlebna; M. - Balas; L. - Bonafos; B. - Pessemesse; L. - Fouret; G. - Vigor; C. - Gaillet; S. - Grober; J. - Bernex; F. - Landrier; J.F. - Kuda; Ondřej - Durand; T. - Coudray; Ch. - Casas; F. - Feillet-Coudray; Ch.
Long-term intake of 9-PAHPA or 9-OAHPA modulates favorably the basal metabolism and exerts an insulin sensitizing effect in obesogenic diet-fed mice
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European Journal of Nutrition. 2021; 60(4); 2013-2027
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IF = 4.868
[ASEP]
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doi
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van der Hoek; M. D. - Nieuwenhuizen; A. G. - Kuda; Ondřej - Bos; P. - Palůchová; Veronika - Verschuren; L. - van den Hoek; A. M. - Kleemann; R. - Veeger; N. J. G. M. - van der Leij; F. R. - Keijer; J.
Intramuscular short-chain acylcarnitines in elderly people are decreased in (pre-)frail females; but not in males
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FASEB Journal. 2020; 34(9); 11658-11671
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IF = 5.191
[ASEP]
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doi
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Kristyna Magno Ondrej Veronika Marina
Speedvac
Growing cells
QTRAP 5500 / SelexION
Growing cells
Eolid Phase Extraction
