Laboratory of Membrane Transport

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Laboratory of Membrane Transport - stazeny soubor 2

Sodium and potassium transporting protein observed in yeast cells through a fluorescence microscope. Green fluorescence corresponds to the protein localized in the cell plasma membrane; red fluorescence visualizes membranes of vacuoles.

About the Laboratory

We study the proteins transporting compounds and signals across the cell membranes. These proteins, called transporters, assure the uptake of nutrients into the cells, efflux of waste compounds from cells and communication with the environment. To study the animal and plant transporters, we mainly use a model eukaryotic microorganism – yeasts. Our research is focused mainly on:

  • Structure, function and regulation of cell transport systems at protein molecule level.
  • Role of transporters in specific cell properties and in diseases.
  • Role of transporters in Candida virulence and pathogenicity.
  • Development of new techniques to estimate cell physiological parameters.

Achievement

Laboratory of Membrane Transport -

Our laboratory is already for many years involved in project Otevřená věda organised by Czech Academy of Sciences. The mission of this successful project is to lure enthusiastic students from secondary schools and show them how amusing the science can be.

Denis Cmunt is one of the first students who started the scientific career in our laboratory with support of Otevřená věda. The link to the inspiring interview with Denis is mentioned below (only in Czech).

http://www.otevrenaveda.cz/cs/aktuality/Veda-mi-poskytuje-prostor-k-rozvoji-sebe-sama-a-prekonavani-vyzev-coz-je-zabava.-Poskytuje-mi-ale-i-moznost-delat-neco-uzitecneho-co-muze-jednou-mit-pozitivni-dopady-na-spolecnost-rika-Denis-Cmunt./?fbclid=IwAR39ZtPtr6IDyUIq1x_BcSiLxxQrVfUhnUV1vbE9_-zYKQINhadcCxnd1to

Natálie Pořízková is another successful student who came to our laboratory via Otevřená věda. Natálie captivated the committee with her work and won the first place in category Live Nature/medical sciences and Biochemistry during the closing conference of Otevřená věda. Here you can read the interview with Natálie (in Czech):

http://www.otevrenaveda.cz/cs/aktuality/Myslim-ze-mi-absolvovani-staze-moc-pomohlo-u-prijimaciho-pohovoru-na-vysokou-skolu-rika-stazistka-Natalie-Porizkova./?fbclid=IwAR2HxBLhTlZ82CqBHSDpy5N1CwiBuX6AKoZ1cmPacAbzGbWXwem7mxVP0qo

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Anne-Sophie Colinet

Successful collaboration with the group of Dr. Pierre Morsomm was crowned by the short-term research fellowship of Belgian student Anne-Sophie Colinet at our department. The aim of joint project is the uncovering of the transport mechanism of Gdp1p in Golgi apparatus of S. cerevisiae. Gdp1 is the Ca2+ transporter homologous to the product of human gene TMEM165. Mutations in this gene are associated with the autosomal recessive disorder Congenital Disorder of Glycosylation, type IIk (CDG II). The main task of Anne-Sophie Colinet was the measurement of the intracellular pH in S. cerevisiae lacking gene encoding the Gdt1 transporter.

On October 1, 2013, our new European project entitled Molecular Mechanisms of Human Fungal Pathogen Host Interaction (ImResFun, FP7-PEOPLE-2013-ITN ) has started. In the consortium composed of nine laboratories and three companies from nine European countries, we will study the interaction of pathogenic yeasts with cells, tissues and organs of the host. Our aim is to identify so far unknown targets in pathogenic yeasts for the development of new antifungal drugs.

Publications

Publication year

Prestigious publication

Search publication

Antunes; M. - Kale; Deepika - Sychrová; Hana - Sá-Correia; I. The Hrk1 kinase is a determinant of acetic acid tolerance in yeast by modulating H+ and K+ homeostasis. Microbial cell. 2023; 10(12); 261-276.

IF = 4.1

Papoušková; Klára - Černá; Karolína - Radová; Viktorie - Zimmermannová; Olga The Role of Cornichons in the Biogenesis and Functioning of Monovalent-Cation Transport Systems. Physiological Research. 2024; 73(Suppl.1); S199-S215.

IF = 1.9

Papoušková; Klára - Zimmermannová; Olga - Sychrová; Hana Distinct regions of its first intracellular loop contribute to the proper localization; transport activity and substrate-affinity adjustment of the main yeast K plus importer Trk1. Biochimica Et Biophysica Acta-Biomembranes. 2024; 1866(7); 184369.

IF = 2.8

Petrisková; L. - Kodedová; Marie - Balážová; M. - Sychrová; Hana - Valachovič; M. Lipid droplets control the negative effect of non-yeast sterols in membranes of Saccharomyces cerevisiae under hypoxic stress. Biochimica Et Biophysica Acta-Molecular and Cell Biology of Lipids. 2024; 1869(7); 159523.

IF = 3.9

Papoušková; Klára - Gómez; M. - Kodedová; Marie - Ramos; J. - Zimmermannová; Olga - Sychrová; Hana Heterologous expression reveals unique properties of Trk K+ importers from nonconventional biotechnologically relevant yeast species together with their potential to support Saccharomyces cerevisiae growth. Yeast. 2023; 40(2); 68-83.

IF = 2.2

Brockhurst; M. - Cavet; J. - Diggle; S. P. - Grainger; D. - Mangenelli; R. - Sychrová; Hana - Martin-Verstraete; I. - Welch; M. - Palmer; T. - Thomas; G.H. Shaping microbiology for 75 years: highlights of research published in Microbiology. Part 2 – Communities and evolution. Microbiology. 2023; 169(6); 001357.

IF = 1.3

People

Head of Laboratory

Head of Laboratory
Tel: 2556
Email: hana.sychrova@fgu.cas.cz

Deputy Head of Laboratory

Tel: 2538
Email: jan.svoboda@fgu.cas.cz

Laboratory staff

Tel: 2557
Email: karolina.cerna@fgu.cas.cz
Associate scientist
Tel: 2419
Email: michala.duskova@fgu.cas.cz
Lab technician
Tel: 2667
Email: pavla.herynkova@fgu.cas.cz
Lab technician
Tel: 2194
Email: kristina.knopfova@fgu.cas.cz
Lab technician
Tel: 2557
Email: mykhailo.streltsov@fgu.cas.cz
Associate scientist
Tel: 2419
Email: klara.papouskova@fgu.cas.cz