Laboratory of Pancreatic Islet Research

Laboratory of Pancreatic Islet Research - bg 3

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Laboratory of Pancreatic Islet Research - rm1g7hz1ph3tkwq9a53j

About the department

Proper function of pancreatic β-cells as glucose sensors throughout life is critical for maintaining glucose homeostasis in the body. Deterioration in the function and quantity of β-cells is fundamental to the development of diabetes. The activity of β-cells is also closely related to the function of other endocrine cells in the pancreatic islets. Although insulin was discovered 100 years ago (1922) and the major signaling pathway leading to insulin secretion has since been described, we still lack many details about the mechanisms of action of pancreatic β-cells and islet cells that would help us prevent and control diabetes, one of the world’s major health problems.

To improve our understanding of how diabetes develops, our lab is studying the details of glucose metabolism in β-cell signaling. We are focusing on:

1. The role of redox signaling and lipid metabolism in β-cells upon glucose stimulation under physiological and pathophysiological conditions.

2. The interplay between β-cells and other endocrine cells in pancreatic islets in response to altered redox status of β-cells.

Our research is performed in a variety of models with diverse level of complexity, ranging from cell cultures to isolated mouse pancreatic islets to whole animals (mice). We use novel experimental strategies in combination with modern omics analyzes.

Laboratory of Pancreatic Islet Research - islets movement

Fluorescent insulin injected into a mouse pancreatic islet

 

Publication


Holendová B, Benáková Š, Křivonosková M, Plecitá-Hlavatá L. Redox Status as a Key Driver of Healthy Pancreatic Beta-Cells. Physiol Res. 2024 Apr 22. Epub ahead of print. PMID: 38647167. IF = 2.103


Holendová, B., Benáková, Š., Křivonosková, M., Pavluch, V., Tauber, J., Gabrielová, E., Ježek, P., & Plecitá-Hlavatá, L. (2024). NADPH oxidase 4 in mouse β cells participates in inflammation on chronic nutrient overload. Obesity (Silver Spring, Md.), 32(2), 339–351. IF = 4.2


Benak, D., Benakova, S., Plecita-Hlavata, L., & Hlavackova, M. (2023). The role of m6A and m6Am RNA modifications in the pathogenesis of diabetes mellitus. Frontiers in endocrinology, 14, 1223583. IF 3.9


Holendova, B., & Plecita-Hlavata, L. (2023). Cysteine residues in signal transduction and its relevance in pancreatic beta cells. Frontiers in endocrinology, 14, 1221520. IF 3.9


Ježek P, Holendová B, Jabůrek M, Dlasková A, Plecitá-Hlavatá L. Contribution of Mitochondria to Insulin Secretion by Various Secretagogues. Antioxid Redox Signal. 2022 May, 36(13-15): 920–952. IF 7.468


Benáková Š, Holendová B, Plecitá-Hlavatá L. Redox Homeostasis in Pancreatic β-Cells: From Development to Failure. Antioxidants (Basel). 2021 Mar 27;10(4):526. IF 7.675


Ježek P, Holendová B, Jabůrek M, Tauber J, Dlasková A, Plecitá-Hlavatá L. The Pancreatic β-Cell: The Perfect Redox System. Antioxidants (Basel). 2021 Jan 29;10(2):197. IF 7.675


Plecitá-Hlavatá L, Jabůrek M, Holendová B, Tauber J, Pavluch V, Berková Z, Cahová M, Schroeder K, Brandes RP, Siemen D, Ježek P.Glucose-stimulated insulin secretion fundamentally requires H2O2 signaling by NADPH oxidase 4. Diabetes. 2020 Jul;69(7):1341-1354. IF 9.461


Plecitá-Hlavatá L, Engstová H, Holendová B, Tauber J, Špaček T, Petrásková L, Křen V, Špačková J, Gotvaldová K, Ježek J, Dlasková A, Smolková K, Ježek P. Mitochondrial Superoxide Production Decreases on Glucose-Stimulated Insulin Secretion in Pancreatic β Cells Due to Decreasing Mitochondrial Matrix NADH/NAD+ Ratio. Antioxid Redox Signal. 2020 Oct 20;33(12):789-815. IF 8.401

Projects

Publikace

Holendová; Blanka - Benáková; Štěpánka - Křivonosková; Monika - Pavluch; Vojtěch - Tauber; Jan - Gabrielová; E. - Ježek; Petr - Plecitá-Hlavatá; Lydie NADPH oxidase 4 in mouse ? cells participates in inflammation on chronic nutrient overload. Obesity. 2024; 32(2); 339-351.

IF = 6.9

People

Head of Laboratory

Vedoucí oddělení
Tel: 2430
Email: lydie.plecita@fgu.cas.cz

Deputy Head of Laboratory

Laboratory staff

Odborný pracovník SŠ, VOŠ - laborantka
Tel: 2447
Email: ludmila.svobodova@fgu.cas.cz
Tel: 2447
Email: Linda.Stokicova@fgu.cas.cz
Tel: 2447
Email: stepanka.benakova@fgu.cas.cz
Odborný pracovník VaV
Tel: 2447
Email: monika.krivonoskova@fgu.cas.cz
Vědecký asistent
Tel: 2430
Email: blanka.holendova@fgu.cas.cz