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Laboratory of Biomaterials and Tissue Engineering

Laboratory of Biomaterials and Tissue Engineering Klinicky užívané cévní protézy vyrobené z expandovaného polytetrafluoroethylénu (ePTFE) a z polyethylén tereftalátu (PET). Materiál protéz je vysoce hydrofobní a neumožňuje rekonstrukci přirozených buněčných vrstev cévní stěny.

What is a tissue replacement?

Irreversible damage to various vitally important tissues and organs, such as blood vessels, heart valves, bones, joints, cartilage or skin, is a serious and frequent consequence of the lifestyle of the contemporary civilized society. It is associated with various metabolic disorders and also with traffic, sport and industrial accidents. Hereditary disorders, wear and aging of the organism also play an adverse role.

When prevention, conservative treatment and surgical interventions fail, it is necessary to replace the damaged tissue. Autologous tissue, i.e. the patient's own tissue, is still considered to be the “gold standard” for tissue substitution. However, autologous tissue is usually available only in limited quantities, and acquiring the tissue burdens the patient with additional surgery and donor site morbidity. For this reason, increasing attention is being paid to artificial materials that serve as scaffolds for reconstructing the damaged tissue.

The main tasks of our laboratory are:

  • to improve currently-used synthetic tissue replacements by introducing cell and other biological components
  • to construct completely new replacements on the basis of synthetic materials and cells
  • to achieve these two  goals, we carry out studies on the molecular mechanisms of cell behavior on synthetic materials, such as the adhesion, growth, differentiation and viability of cells on artificial materials, together with studies on potential damage and immune activation.

Projects

Achievements

Skin tissue engineering

Construction of the bilayer structure of human keratinocytes (the main cell type of the epidermis) and fibroblasts (the main cell type of the dermis) to nanofiber membranes of polylactide. More

Publications

Zárubová; Jana - Hasani-Sadrabadi; M. M. - Dashtimoghadam; E. - Zhang; X. - Ansari; S. - Li; S. - Moshaverinia; A. Engineered Delivery of Dental Stem-Cell-Derived Extracellular Vesicles for Periodontal Tissue Regeneration . Advanced Healthcare Materials. 2022; 11(12)); 2102593 . IF = 11.092 [ASEP] [ doi ]
Zárubová; Jana - Hasani-Sadrabadi; M. M. - Ardehali; R. - Li; S. Immunoengineering strategies to enhance vascularization and tissue regeneration . Advanced Drug Delivery Reviews. 2022; 184(May)); 114233 . IF = 17.873 [ASEP] [ doi ]
Vackova; Irena - Vavřínová; E. - Musílková; Jana - Havlas; V. - Petrenko; Yuriy . Hypothermic Storage of 3D Cultured Multipotent Mesenchymal Stromal Cells for Regenerative Medicine Applications . Polymers. 2022; 14(13)); 2553 . IF = 4.967 [ASEP] [ doi ]
Novotný; T. - Eckhardt; Adam - Doubková; Martina - Knitlová; Jarmila - Vondrášek; David - Vanaskova; E. - Ošťádal; M. - Uhlík; J. - Bačáková; Lucie - Musílková; Jana . The possible role of hypoxia in the affected tissue of relapsed clubfoot . Scientific Reports. 2022; 12(1)); 4462 . IF = 4.996 [ASEP] [ doi ]
Neubertová; V. - Kasálková-Slepičková; N. - Vokatá; B. - Bačáková; Lucie - Švorčík; V. - Kolská; Z. Influence of UV irradiation and subsequent chemical grafting on the surface properties of cellulose . Cellulose. 2022; 29(3); 1405-1418 . IF = 6.123 [ASEP] [ doi ]
Němčáková; Ivana - Litvinec; Andrej - Mandys; V. - Potocký; Štěpán - Plencner; Martin - Doubková; Martina - Naňka; O. - Olejníčková; Veronika - Šaňková; Barbora - Bartoš; M. - Ukraintsev; E. - Babčenko; O. - Bačáková; Lucie - Kromka; Alexander - Rezek; B. - Sedmera; David . Coating Ti6Al4V implants with nanocrystalline diamond functionalized with BMP-7 promotes extracellular matrix mineralization in vitro and faster osseointegration in vivo . Scientific Reports. 2022; 12(1)); 5264 . IF = 4.996 [ASEP] [ doi ]
Klimek; K. - Benko; A. - Vandrovcová; Marta - Trávníčková; Martina - Douglas; T. E. L. - Tarczynska; M. - Brož; Antonín - Gaweda; K. - Ginalska; G. - Bačáková; Lucie . Biomimetic biphasic curdlan-based scaffold for osteochondral tissue engineering applications Characterization and preliminary evaluation of mesenchymal stem cell response in vitro . Biomaterials Advances. 2022; 135(April)); 212724 . IF = 8.457 [ASEP] [ doi ]
Kacvinská; K. - Trávníčková; Martina - Vojtová; L. - Poláček; P. - Dorazilová; J. - Kohoutek; M. - Hlináková; K. - Pajorová; Julia - Tirpáková; Maria - Bačáková; Lucie . Porous cellulose-collagen scaffolds for soft tissue regeneration: influence of cellulose derivatives on mechanical properties and compatibility with adipose-derived stem cells . Cellulose. 2022; 29(15); 8329-8351 . IF = 6.123 [ASEP] [ doi ]
Chlupáč; Jaroslav - Matějka; R. - Koňařík; M. - Novotný; R. - Šimůnková; Z. - Mrázová; I. - Fabian; O. - Zapletal; M. - Pulda; Z. - Lipenský; J. F. - Štěpanovská; J. - Hanzálek; K. - Brož; Antonín - Novák; T. - Lodererová; A. - Voska; L. - Adla; T. - Froněk; J. - Rozkot; M. - Forostyak; S. - Kneppo; P. - Bačáková; Lucie - Pirk; J. Vascular Remodeling of Clinically Used Patches and Decellularized Pericardial Matrices Recellularized with Autologous or Allogeneic Cells in a Porcine Carotid Artery Model . International Journal of Molecular Sciences. 2022; 23(6)); 3310 . IF = 6.208 [ASEP] [ doi ]
Gabor; R. - Cvrček; L. - Doubková; Martina - Nehasil; V. - Hlinka; J. - Unucka; P. - Buřil; M. - Podepřelová; A. - Seidlerová; J. - Bačáková; Lucie . Hybrid coatings for orthopaedic implants formed by physical vapour deposition and microarc oxidation . Materials and Design. 2022; 219(July)); 110811 . IF = 9.417 [ASEP] [ doi ]
Dubišová; J. - Svobodová Buriánová; J. - Svobodová; Lucie - Makovický; P. - Martinez-Varea; N. - Cimpean; A. - Fawcett; J. W. - Kwok; J. C. F. - Kubinová; Šárka . Oral treatment of 4-methylumbelliferone reduced perineuronal nets and improved recognition memory in mice . Brain Research Bulletin. 2022; 181(Apr); 144-156 . IF = 3.715 [ASEP] [ doi ]
Dodda; J. M. - Azar; M. G. - Bělský; P. - Šlouf; Miroslav - Brož; Antonín - Bačáková; Lucie - Kadlec; J. - Remiš; T. Biocompatible hydrogels based on chitosan; cellulose/starch; PVA and PEDOT:PSS with high flexibility and high mechanical strength . Cellulose. 2022; 29(12); 6697-6717 . IF = 6.123 [ASEP] [ doi ]
Bačáková; Lucie - Novotná; Katarína - Hadraba; Daniel - Musílková; Jana - Slepička; P. - Beran; M. Influence of Biomimetically Mineralized Collagen Scaffolds on Bone Cell Proliferation and Immune Activation . Polymers. 2022; 14(3)); 602 . IF = 4.967 [ASEP] [ doi ]
Zasońska; Beata Anna - Brož; Antonín - Šlouf; Miroslav - Hodan; Jiří - Petrovský; Eduard - Hlídková; Helena - Horák; Daniel . Magnetic superporous poly(2-hydroxyethyl methacrylate) hydrogel scaffolds for bone tissue engineering . Polymers. 2021; 13(11); 1-13); 1871 . IF = 4.967 [ASEP] [ doi ]
Vandrovcová; Marta - Tolde; Z. - Vaněk; Přemysl - Nehasil; V. - Doubková; Martina - Trávníčková; Martina - Drahokoupil; Jan - Buixaderas; Elena - Borodavka; Fedir - Nováková; J. - Bačáková; Lucie . Beta-Titanium Alloy Covered by Ferroelectric Coating-Physicochemical Properties and Human Osteoblast-Like Cell Response . Coatings. 2021; 11(2)); 210 . IF = 3.236 [ASEP] [ doi ]
Trávníčková; Martina - Kasálková-Slepičková; N. - Sedlář; Antonín - Molitor; M. - Musílková; Jana - Slepička; P. - Švorčík; V. - Bačáková; Lucie . Differentiation of adipose tissue-derived stem cells towards vascular smooth muscle cells on modified poly(L-lactide) foils . Biomedical Materials. 2021; 16(2)); 025016 . IF = 4.103 [ASEP] [ doi ]
Štěpanovská; J. - Šupová; Monika - Hanzálek; K. - Brož; Antonín - Matějka; R. Collagen Bioinks for Bioprinting: A Systematic Review of Hydrogel Properties; Bioprinting Parameters; Protocols; and Bioprinted Structure Characteristics . Biomedicines. 2021; 9(9)); 1137 . IF = 4.757 [ASEP] [ doi ]
Steinerová; Marie - Matějka; Roman - Štěpanovská; Jana - Filová; Elena - Staňková; Ľubica - Rysová; M. - Martinová; L. - Dragounová; Helena - Domonkos; Mária - Artemenko; Anna - Babchenko; Oleg - Otáhal; M. - Bačáková; Lucie - Kromka; Alexander . Human osteoblast-like SAOS-2 cells on submicron-scale fibers coated with nanocrystalline diamond films . Materials Science & Engineering C-Materials for Biological Applications. 2021; 121(Feb)); 111792 . IF = 8.457 [ASEP] [ doi ]
Sedlář; Antonín - Trávníčková; Martina - Matějka; Roman - Pražák; Šimon - Mészáros; Zuzana - Bojarová; Pavla - Bačáková; Lucie - Křen; Vladimír - Slámová; Kristýna . Growth Factors VEGF-A(165) and FGF-2 as Multifunctional Biomolecules Governing Cell Adhesion and Proliferation . International Journal of Molecular Sciences. 2021; 22(4)); 1843 . IF = 6.208 [ASEP] [ doi ]
Sedlář; Antonín - Trávníčková; Martina - Bojarová; Pavla - Vlachová; Miluše - Slámová; Kristýna - Křen; Vladimír - Bačáková; Lucie . Interaction between Galectin-3 and Integrins Mediates Cell-Matrix Adhesion in Endothelial Cells and Mesenchymal Stem Cells . International Journal of Molecular Sciences. 2021; 22(10)); 5144 . IF = 6.208 [ASEP] [ doi ]
Sailapu; S. K. - Blanquer; Andreu - Duch; M. - Esquivel; J. P. - Nogués; C. - Esteve; J. - Sabaté; N. Self-activated microbatteries to promote cell death through local electrical stimulation . Nano Energy. 2021; 83(May)); 105852 . IF = 19.069 [ASEP] [ doi ]
Pilná; H. - Hájková; V. - Knitlová; Jarmila - Lišková; Jana - Elsterová; Jana - Melková; Z. Vaccinia Virus Expressing Interferon Regulatory Factor 3 Induces Higher Protective Immune Responses against Lethal Poxvirus Challenge in Atopic Organism . Viruses. 2021; 13(10)); 1986 . IF = 5.818 [ASEP] [ doi ]
Novotná; Z. - Kolská; Z. - Slepička; P. - Kasálková-Slepičková; N. - Fajstavr; D. - Bačáková; Lucie - Švorčík; V. Polymery pro tkáňové inženýrství . Chemické listy. 2021; 115(12); 634-643 . IF = 0.356 [ASEP]
Molitor; M. - Trávníčková; Martina - Měšťák; O. - Christodoulou; P. - Sedlář; Antonín - Bačáková; Lucie - Lucchina; S. The Influence of Low- and High-Negative-Pressure Liposuction and Different Harvesting Sites on the Viability and Yield of Adipocytes and Other Nucleated Cells . Aesthetic Plastic Surgery. 2021; 45(6); 2952-2970 . IF = 2.708 [ASEP] [ doi ]
Molitor; M. - Trávníčková; Martina - Měšťák; O. - Christodoulou; P. - Sedlář; Antonín - Bačáková; Lucie - Lucchina; S. The Influence of High and Low Negative Pressure Liposuction and Various Harvesting Techniques on the Viability and Function of Harvested Cells-a Systematic Review of Animal and Human Studies . Aesthetic Plastic Surgery. 2021; 45(5); 2379-2394 . IF = 2.708 [ASEP] [ doi ]

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People

Doc. MUDr. Lucie Bačáková, CSc.
Head of the Laboratory

Mgr. Elena Filová, Ph.D.
Deputy Head of the Laboratory

 

Ing. Irena Vacková, CSc.
Researcher

Věra Lisá, prom. biol.
Researcher
Mgr. Jana Racková (nee. Lišková), Ph.D.
Researcher (maternity leave)
RNDr. Jana Musílková, CSc.
Researcher
RNDr. Lubica Staňková (nee. Grausová), Ph.D.
Researcher
RNDr. Lucie Svobodová, Ph.D.
Researcher

Mgr. Antonín Brož, Ph.D.
Researcher

Ing. Markéta Zikmundová, Ph.D.
Postdoctoral Researcher (maternity leave)

 

MUDr. Jaroslav Chlupáč, Ph.D.
Postdoctoral Researcher
Mgr. Ivana Němčáková (nee. Kopová), Ph.D.
Postdoctoral Researcher (maternity leave)
Ing. Jana Zárubová (nee. Havlíková), Ph.D.
Postdoctoral Researcher
MUDr. Martina Trávníčková, Ph.D.
Postdoctoral Researcher
Mgr. Martina Doubková
PhD Student

Mgr. Júlia Pajorová
PhD Student

Mgr. Antonín Sedlář
PhD Student

Mgr. Šimon Pražák
PhD Student

  MSc. Marina Malić
PhD Student
  MSc. Yu-Chieh Wu
PhD Student
Mgr. Jarmila Knitlová
PhD Student
 

Ing. Roman Matějka
Research Assistant

 

Ing. Jana Štěpanovská
Research Assistant

Jana Voborníková
Administrative Worker
Ivana Zajanová
Technician
  Bc. Kateřina Pokorná
MSc. student