Laboratory of Biomaterials and Tissue Engineering

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Laboratory of Biomaterials and Tissue Engineering - stazeny soubor 15

About the Laboratory

What is a tissue replacement?

Irreversible damage to various vitally important tissues and organs, such as blood vessels, heart valves, bones, joints, cartilage or skin, is a serious and frequent consequence of the lifestyle of the contemporary civilized society. It is associated with various metabolic disorders and also with traffic, sport and industrial accidents. Hereditary disorders, wear and aging of the organism also play an adverse role.

When prevention, conservative treatment and surgical interventions fail, it is necessary to replace the damaged tissue. Autologous tissue, i.e. the patient’s own tissue, is still considered to be the “gold standard” for tissue substitution. However, autologous tissue is usually available only in limited quantities, and acquiring the tissue burdens the patient with additional surgery and donor site morbidity. For this reason, increasing attention is being paid to artificial materials that serve as scaffolds for reconstructing the damaged tissue.

The main tasks of our laboratory are:

  • to improve currently-used synthetic tissue replacements by introducing cell and other biological components
  • to construct completely new replacements on the basis of synthetic materials and cells
  • to achieve these two goals, we carry out studies on the molecular mechanisms of cell behavior on synthetic materials, such as the adhesion, growth, differentiation and viability of cells on artificial materials, together with studies on potential damage and immune activation.

Achievements

 

Laboratory of Biomaterials and Tissue Engineering -

Differentiated cells grow slower and have a limited viability in cell culture conditions. Isolation of differentiated cells from the patient does not eliminate the problem with other additive operations and damage of the other tissues of the patient. For example, for obtaining endothelial or the smooth muscle cells for reconstruction of blood vessels must remove part of subcutaneous veins from the patient. Stem cells from the patient’s body can be obtained still more simply, e.g. blood sampling (endothelial progenitor cells), puncture bone marrow of the bone tissue under the skin, e.g. from the hip bone, liposuction of fat tissue. Therefore, in collaboration with Hospital Motol we try to differentiate adipose stem cells into vascular or bone cells for the colonization of our constructs.

Adipose Stem Cells_Bacakova_FgU_December7.pdf

Construction of the bilayer structure of human keratinocytes (the main cell type of the epidermis) and fibroblasts (the main cell type of the dermis) to nanofiber membranes of polylactide.

Laboratory of Biomaterials and Tissue Engineering - 3 zahajeni inzenyrstvi koznich bunek

Bačáková M, Musílková J, Riedel T, Stránská D, Brynda E, Žaloudková M, Bačáková L. The potential applications of fibrin-coated electrospun polylactide nanofibers in skin tissue engineering. International Journal of Nanomedicine. Roč. 11 (2016): 771-789, 2016, IF = 4.320

Bačáková M, Lopot F, Hadraba D, Varga M, Žaloudková M, Stránská D, Suchý T, Bačáková L. Effects of fiber density and plasma modification of nanofibrous membranes on the adhesion and growth of HaCaT keratinocytes. Journal of Biomaterials Applications. Vol. 29 (6): 837-853, 2015,IF = 1.988

 

Publications

Staňková; Ľubica - Kutová; A. - Doubková; Martina - Kvítek; O. - Vokatá; B. - Sedlář; Antonín - Idriss; H. - Slepička; P. - Švorčík; V. - Bačáková; Lucie Argon plasma-modified bacterial nanocellulose: Cell-specific differences in the interaction with fibroblasts and endothelial cells. Carbohydrate Polymer Technologies and Applications. 2024; 7(June); 100470.

IF = 6.2

Vagnerová; Karla - Hudcovic; Tomáš - Vodička; Martin - Ergang; Peter - Klusoňová; Petra - Hermanová; Petra - Šrůtková; Dagmar - Pácha; Jiří The effect of oral butyrate on colonic short-chain fatty acid transporters and receptors depends on microbial status. Frontiers in Pharmacology. 2024; 15(Mar 26); 1341333.

IF = 4.4

Štěpánková; K. - Mareková; D. - Kubášová; K. - Sedláček; R. - Turnovcová; K. - Vacková; Irena - Kubinová; Šárka - Makovický; P. - Petrovičová; M. - Kwok; J. C. F. - Jendelová; P. - Urdzíková; L. 4-Methylumbeliferone Treatment at a Dose of 1.2 g/kg/Day Is Safe for Long-Term Usage in Rats. International Journal of Molecular Sciences. 2023; 24(4)); 3799.

IF = 5.6

Rogulska; Olena - Havelková; Jarmila - Petrenko; Yuriy Cryopreservation of Organoids. Cryo-letters. 2023; 44(2); 65-75.

IF = 1.0

Brockhurst; M. - Cavet; J. - Diggle; S. P. - Grainger; D. - Mangenelli; R. - Sychrová; Hana - Martin-Verstraete; I. - Welch; M. - Palmer; T. - Thomas; G.H. Shaping microbiology for 75 years: highlights of research published in Microbiology. Part 2 – Communities and evolution. Microbiology. 2023; 169(6); 001357.

IF = 2.8

Trávníčková; Martina - Filová; Elena - Slepička; P. - Kasálková-Slepičková; N. - Kocourek; Tomáš - Žaloudková; Margit - Suchý; Tomáš - Bačáková; Lucie Titanium-Doped Diamond-like Carbon Layers as a Promising Coating for Joint Replacements Supporting Osteogenic Differentiation of Mesenchymal Stem Cells. International Journal of Molecular Sciences. 2024; 25(5); 2837.

IF = 5.6

Photogallery

People

Head of Laboratory

Tel: 3743
Email: lucie.bacakova@fgu.cas.cz

Deputy Head of Laboratory

Laboratory staff

Vědecký pracovník
Tel: 3765
Email: antonin.broz@fgu.cas.cz
Odborný pracovník VaV
Tel: 3743
Email: jaroslav.chlupac@fgu.cas.cz
Vědecký pracovník
Tel: 3765
Email: martina.doubkova@fgu.cas.cz
Vědecký pracovník
Tel: 3742
Email: elena.filova@fgu.cas.cz
Tel: 3765
Email: terezie.futoova@fgu.cas.cz
Tel: 3506
Email: jarmila.havelkova@fgu.cas.cz
Odborný pracovník SŠ, VOŠ - laborantka
Tel: 3742
Email: andrea.hejdova@fgu.cas.cz
Odborný pracovník Vav
Tel: 3743
Email: jarmila.knitlova@fgu.cas.cz
Odborný pracovník Vav
Tel: 3745
Email: vera.lisa@fgu.cas.cz
Doktorand
Tel: 3765
Email: marina.malic@fgu.cas.cz
Vědecký pracovník
Tel: 3742
Email: jana.musilkova@fgu.cas.cz
Vědecký asistent
Tel: 3741
Email: ivana.nemcakova@fgu.cas.cz
Odborný pracovník VaV
Tel: 3743
Email: yuriy.petrenko@fgu.cas.cz
Odborný pracovník VaV
Tel: 3765
Email: simon.prazak@fgu.cas.cz
Odborný pracovník SŠ, VOŠ - laborantka
Tel: 3743
Email: adriena.pritasilova@fgu.cas.cz
Vědecký asistent
Tel: 3742
Email: jana.rackova@fgu.cas.cz
Vědecký asistent
Email: olena.rohulska@fgu.cas.cz
Tel: 3741
Email: antonin.sedlar@fgu.cas.cz
Vědecký pracovník
Tel: 3765
Email: lubica.stankova@fgu.cas.cz
Vědecký asistent
Tel: 3741
Email: lucie.svobodova@fgu.cas.cz
Tel: 3743
Email: Julia.Tomsu@fgu.cas.cz
Tel: 3741
Email: Martina.Travnickova@fgu.cas.cz
Vědecký pracovník
Tel: 3743
Email: irena.vackova@fgu.cas.cz
Odborný pracovník VaV
Tel: 3743
Email: yuchieh.wu@fgu.cas.cz
Laborantka
Tel: 3744
Email: ivana.zajanova@fgu.cas.cz
Vědecký asistent
Tel: 3509
Email: jana.zarubova@fgu.cas.cz
Tel: 3765
Email: marketa.zikmundova@fgu.cas.cz