Tomocube’s holotomography (HT) technology enable users to quantitatively and noninvasively investigate live biological cells and thin tissues in 3D. HT reconstructs the 3D refractive index (RI) distribution of live cells and provides structural and chemical information about the cell, including dry mass, morphology and dynamics of the cellular membrane. This can be done very easily and quickly, because RI is an intrinsic optical parameter of a material and thus HT does not require any sample preparation.
Tomocube’s HT-2 offers advantage of correlative 3D fluorescence and embedded deconvolution for confocal like images. While HT enables quantitative analysis (volume, surface area, mean RI, concentration and dry mass) and label-free visualization of live cell samples, fluorescence microscopy provides the sometimes-essential molecular specificity. Combining the two technologies together provides the best of both worlds — imaging longer with minimal phototoxicity and photobleaching using HT and intermittent fluorescence.
Advantage of the machine – no labeling, high resolution, fast imaging, low phototoxicity, providing stable culture environment etc.
For more information, please visit: https://www.tomocube.com/products/ht2h
