Intranet

INSTITUTE OF PHYSIOLOGY CAS

Cutting-edge science for health

Laboratory of Adipose Tissue Biology

Laboratory of Adipose Tissue Biology

The Laboratory of Adipose Tissue Biology (Laboratory) is studying physiological regulations of metabolisms and their disturbances in obesity and associated diseases (i.e. Metabolic syndrome). In order to help treat these disorders we explore the influence of drugs, diet and natural substances, namely n-3 polyunsaturated fatty acids of marine origin (omega-3). Our results show the importance of the metabolism of adipose tissue for accumulation of body fat and reveal new possibilities in the prevention and treatment of metabolic disorders linked to obesity by modulating adipose tissue metabolism. We investigate mechanisms that regulate metabolism in health and disease by combining experiments on mice and cell models with clinical studies, and we try to apply new knowledge in clinical medicine. We also focus on ontogenetic development of the studied mechanisms. While working with the manufacturers of the tested substances in the Czech Republic and Norway, we investigate the possible use of omega-3 to increase the effect of drugs and other substances in the treatment of selected diseases. Research projects of the Laboratory reflect the current grant support and the existence of research units established at the Laboratory during 2015.

The Energy Metabolism Unit (PI: J. Kopecký) is focused on two complementary areas (i) systemic effects of intrinsic metabolism of white adipose tissue (WAT), and (ii) developmental aspects of energy metabolism and perinatal development in general. With respect to the WAT metabolism, we aim to understand the importance of a "futile" cycle involving the triglyceride hydrolysis and re-esterification of fatty acids (triglyceride/fatty acid cycling) in adipocytes of WAT for resistance to obesity and associated metabolic disorders, namely in the context of calorie restriction, omega-3 administration and cold exposure. The influence of the above manipulations on the formation of lipid mediators, mitochondrial metabolism and proliferation of cells in the adipose tissue is also being characterized. We also focus on the role of epicardial WAT metabolism in heart failure and assocciated cachexia in human patients. Regarding the developmental studies, we focus on the control of postnatal changes in muscle energy metabolism and its significance to obesity in mice, and we characterize perinatal changes in transcriptome of WAT, muscle and liver using a unique biobank of autopsy samples collected from human fetuses and newborns. Core members (without workers on maternity leave): P. Janovska, K. Bardova, P. Zouhar, K. Adamcova, J. Funda, L. Lenkova, I. Irodenko, D. Salkova.

The Glucose Homeostasis Unit (PI: M. Rossmeisl), investigates the mechanisms associated with the effect of dietary lipids on metabolism, with a special focus on omega-3 fatty acids and different lipid forms of their administration. Specifically, we study the changes in ectopic lipid accumulation, glucose metabolism and insulin sensitivity in response to dietary supplementation with omega-3 using various mouse models of diet-induced obesity and non-alcoholic fatty liver disease. We also focus on insulin-sensitizing effects of omega-3 administered as triacylglycerols, phospholipids (Krill oil) or wax esters (Calanus oil), which are studied at the whole-body as well as organ level (muscle, adipose tissue, liver) using in vivo technique of hyperinsulinemic-euglycemic clamps. Collaboration with our Laboratory's Metabolomics Unit (now an independent laboratory, see below) allows us to study the effects of omega-3 supplementation on lipidome, with a particular focus on endocannabinoids. Translational potential regarding the beneficial effects of omega-3 on metabolism is also tested using the transgenic mice expressing the human version of the transcription factor PPARalpha. Relatively recently, we have also begun to address changes in intestinal metabolism in the context of the administration of omega-3 or drugs (e.g., metformin). Core members (without workers on maternity leaf): O. Horakova, V. Kalendova, G. Sistilli, A. Krejcarkova, K. Sedova.

 

All the above mentioned laboratories are closely collaborating and are engaged in translational research conducted in collaboration with clinical as well as industrial partners.

 

Until 2019, also the Metabolomics Unit (PI: O. Kuda) formed a part of the Laboratory. However, this research unit became an independent Laboratory of Metabolism of Bioactive Lipids at the Institute, based on the "Lumina Queruntur praemium", a prestigious prize awarded by the Czech Academy of Sciences to O. Kuda. Thus, also M. BrezinovaM. Oseeva, V. Paluchova and M. Rombaldova moved to the new Kuda's laboratory.

 

Projects

The role of intestine in the metabolic effects of dietary supplementation with omega-3 fatty acids

Dietary supplementation with Omega-3 such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) may protect against obesity while exerting hypolipidemic and anti-inflammatory effects. While the effects of omega-3 on lipid metabolism in the liver are relatively well known, changes in intestinal metabolism due to omega-3 administration have not yet been explored in detail. Our previous studies showed that metabolic effects are stronger when omega-3 are administered as phospholipids (Rossmeisl et al., PlosOne 2012). The main goal of this project is to identify the nature of metabolic effects of omega-3 administered in different lipid forms (triacylglycerols, phospholipids, wax esters), mainly with regard to the possible involvement of the intestine in these effects.   More

Physiological relevance of white adipose tissue plasticity and its relationship to the development of obesity and to thermogenic response

Sufficient plasticity of white adipose tissue allows to adjust appropriately various metabolic processes in response to changing environmental conditions. Metabolic plasticity of adipose tissue is linked with changes in the size and content of cells within the tissue. A hypothesis will be verified that adipose tissue plasticity, namely the proliferative potential of adipose tissue cells, represents an important factor counteracting toxic effects of fatty acids that are released during the breakdown of lipid stores. Reduced ability of the organism to activate these processes could contribute to the  development of obesity and its metabolic consequences. More

The effect of exercise training and omega-3 supplementation on metabolic parameters and lipidome in elderly women

In the elderly, lifestyle interventions based on the increased physical activity are primarily aimed to improve muscle function and/or cardiovascular fitness, but recent data suggest that adipose tissue may also contribute to the beneficial effects of exercise on metabolism, inflammation and overall health. Wax esters, contained in Calanus oil, is one of the new lipid forms of Omega-3, which may exert improved efficacy in terms of metabolic effects. Therefore, the aim of this project is to study in sedentary older women the effects of exercise training alone or in combination with Calanus oil supplementation. More

Changes of transcriptome during early postnatal development in humans: impact of premature birth on control of energy metabolism

Premature birth and its complications are the number one cause of death among children under 5 years of age and could impose long-term developmental and health problems. To improve the prognosis of premature newborns, it is necessary to understand the causes of their insufficient postnatal adaptation that depends on the changes in energy metabolism and other mechanisms. However, this research is limited by the lack of clinical materials.  More

Achievements

Our laboratory as a partner in EU projects

Our laboratory has been a partner in EU projects supported through Cooperation program of the European Community (FP7 and H2020) – BIOCLAMS (2010–2015), DIABAT (2011–2015), and FOIE GRAS. More

Our articles repeatedly on the list of the most significant results of Czech Academy of Sciences

Set of our papers regarding novel possibilities of the use of sea fish lipids for prevention and treatment of obesity and associated disorders was included on the list of most significant results of Czech Academy of Sciences in 2009. Our later article dedicated to the combination of omega-3 and mild calorie restriction was included on the same list in 2011. Furthermore, our results describing the changes in adipose tissue metabolism during cold exposure and their relevance to susceptibility to obesity were listed among the best achievements of the Czech Academy of Sciences in 2017.    More

Publications

Zouhar; Petr - Rakipovski; G. - Bokhari; M. H. - Busby; O. - Paulsson; J. F. - Conde-Frieboes; K. W. - Fels; J. J. - Raun; K. - Andersen; B. - Cannon; B. - Nedergaard; J. UCP1-independent glucose-lowering effect of leptin in type 1 diabetes: only in conditions of hypoleptinemia . AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM. 2020; 318(1); E72-E76 . IF = 3.469 [ASEP] [ doi ]
Pomar; C. A. - Kuda; Ondřej - Kopecký; Jan - Rombaldová; Martina - Castro; H. - Picó; C. - Sánchez; J. - Palou; A. Maternal diet; rather than obesity itself; has a main influence on milk triacylglycerol profile in dietary obese rats . Biochimica Et Biophysica Acta-Molecular and Cell Biology of Lipids. 2020; 1865(2)); 158556 . IF = 4.519 [ASEP] [ doi ]
Palůchová; Veronika - Vik; A. - Čajka; Tomáš - Březinová; Marie - Brejchová; Kristýna - Bugajev; Viktor - Dráberová; Lubica - Dráber; Peter - Burešová; Jana - Kroupová; Petra - Bardová; Kristina - Rossmeisl; Martin - Kopecký; Jan - Hansen; T. V. - Kuda; Ondřej . Triacylglycerol-Rich Oils of Marine Origin are Optimal Nutrients for Induction of Polyunsaturated Docosahexaenoic Acid Ester of Hydroxy Linoleic Acid (13-DHAHLA) with Anti-Inflammatory Properties in Mice . Molecular Nutrition & Food Research. 2020; 64(11)); 1901238 . IF = 5.309 [ASEP] [ doi ]
Palůchová; Veronika - Oseeva; Marina - Březinová; Marie - Čajka; Tomáš - Bardová; Kristina - Adamcová; Kateřina - Žáček; Petr - Brejchová; Kristýna - Balas; L. - Chodounská; Hana - Kudová; Eva - Schreiber; R. - Zechner; R. - Durand; T. - Rossmeisl; Martin - Abumrad; N. A. - Kopecký; Jan - Kuda; Ondřej . Lipokine 5-PAHSA is Regulated by Adipose Triglyceride Lipase and Primes Adipocytes for de novo Lipogenesis in Mice . Diabetes. 2020; 69(3); 300-312 . IF = 7.720 [ASEP] [ doi ]
Burešová; Jana - Janovská; Petra - Kuda; Ondřej - Křížová; J. - Romijnders-van der Stelt; I. - Keijer; J. - Hansíková; H. - Rossmeisl; Martin - Kopecký; Jan . Postnatal induction of muscle fatty acid oxidation in mice differing in propensity to obesity: a role of pyruvate dehydrogenase . International Journal of Obesity. 2020; 44(1); 235-244 . IF = 4.419 [ASEP] [ doi ]

Article photogallery

The entire photogallery

People

MUDr. Martin Rossmeisl, Ph.D.

Head of the Laboratory

MUDr. Jan Kopecký, DrSc.

Deputy Head of the Laboratory

Mgr. Olga Horáková, Ph.D.

Senior Researcher

Ing. Petra Janovská, Ph.D.

Senior Researcher

Mgr. Kristina Bardová, Ph.D.

Junior Researcher 

RNDr. Petr Zouhar, Ph.D.

Junior Researcher 

Mgr. Kateřina Adamcová

PhD Student

Mgr. Jiří Funda

PhD Student

Mgr. Veronika Kalendová

PhD Student

Ing. Lucie Leňková

PhD Student

Mgr. Gabriela Sistilli

PhD Student

Bc. Ilariia Irodenko

Student

Mgr. Karolína Seďová

Technician

Jana Jahelková

Technician

Ing. Adéla Krejcárková, Ph.D.

Technician

Daniela Šálková

Technician